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Interaction of S413-PV cell penetrating peptide with model membranes: relevance...
Mano, Miguel; Henriques, Ana; Paiva, Artur; Prieto, Manuel; Gavilanes, Francisco; Simões, Sérgio; Lima, Maria C. Pedroso DeCell penetrating peptides (CPPs) have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest both in vitro and in vivo, although the mechanisms by which the cellular uptake occurs remain unclear and controversial. Following our previous work demonstrating that the cellular uptake of the S413-PV CPP occurs mainly through an endocytosis-independent ...
Thermostability of cardosin A from Cynara cardunculus L.
Pina, David G.; Oliveira, Claudia S.; Sarmento, Ana C.; Barros, Marlene; Pires, Euclides; Zhadan, Galina G.; Villar, Enrique; Gavilanes, FranciscoThe structural stability of cardosin A, a plant aspartic proteinase (AP) from Cynara cardunculus L., has been investigated by high-sensitivity differential scanning calorimetry, intrinsic fluorescence and circular dichroism spectroscopy, and enzymatic activity assays. Even though the thermal denaturation of cardosin A is partially irreversible, valid thermodynamic data can be obtained within a wide pH region. A...
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