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Purification, crystallization and preliminary X-ray diffraction analysis of ade...
Gavel, Olga Yu.; Kladova, Anna V.; Bursakov, Sergey A.; Dias, João M.; Texeira, Susana; Moura, José J. G.; Moura, Isabel; Romão, Maria J.; Trincão, JoséActa Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jul 1;64(Pt 7):593-5 ; Native zinc/cobalt-containing ATP sulfurylase (ATPS; EC 2.7.7.4; MgATP: sulfate adenylyltransferase) from Desulfovibrio desulfuricans ATCC 27774 was purified to homogeneity and crystallized. The orthorhombic crystals diffracted to beyond 2.5 A ° resolution and the X-ray data collected should allow the determination of the structure o...
Structural stability of adenylate kinase from the sulfate-reducing bacteria Des...
Moura, José J. G.; Moura, Isabel; Gavel, Olga Yu.; Bursakov, Sergey A.; Pina, David G.; Zhadan, Galina G.; Shnyrov, Valery L.Biophysical Chemistry 110 (2004) 83–92 ; A novel adenylate kinase (AK) has recently been purified from Desulfovibrio gigas and characterized as a Co2+/Zn2+-containing enzyme: this is an unusual characteristic for AKs from Gram-negative bacteria, in which these enzymes are normally devoid of metals. Here, we studied the conformational stability of holo- and apo-AK as a function of temperature by differential sc...
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