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Hepatic UDP-glucose 13C isotopomers from [U-13C]glucose: A simple analysis by 1...
Mendes, Ana C.; Caldeira, M. Madalena; Silva, Cláudia; Burgess, Shawn C.; Merritt, Matthew E.; Gomes, Filipe; Barosa, Cristina; Delgado, Teresa C.Menthol glucuronide was isolated from the urine of a healthy 70-kg female subject following ingestion of 400 mg of peppermint oil and 6 g of 99% [U-13C]glucose. Glucuronide 13C-excess enrichment levels were 4-6% and thus provided high signal-to-noise ratios (SNRs) for confident assignment of 13C-13C spin-coupled multiplet components within each 13C resonance by 13C NMR. The [U-13C]glucuronide isotopomer derived...
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear m...
Jin, Eunsook S.; Jones, John G.; Burgess, Shawn C.; Merritt, Matthew E.; Sherry, A. Dean; Malloy, Craig R.A recently introduced tracer, [3,4-13C2] glucose, was compared to the widely used tracer, [6,6-2H2]glucose, for measurement of whole-body glucose turnover. The rate of glucose production (GP) was measured in rats after primed infusions of [3,4-13C2]glucose, [6,6-2H2]glucose, or both tracers simultaneously followed by a constant infusion of tracer(s) over 90 min. Blood glucose was purified and converted into mon...
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxe...
Jin, Eunsook S.; Jones, John G.; Merritt, Matthew; Burgess, Shawn C.; Malloy, Craig R.; Sherry, A. DeanA triple-tracer method was developed to provide absolute fluxes contributing to endogenous glucose production and hepatic tricarboxylic acid (TCA) cycle fluxes in 24-h-fasted rats by 2H and 13C nuclear magnetic resonance (NMR) analysis of a single glucose derivative. A primed, intravenous [3,4-13C2]glucose infusion was used to measure endogenous glucose production; intraperitoneal 2H2O (to enrich total body wat...
Noninvasive evaluation of liver metabolism by 2H and 13C NMR isotopomer analysi...
Burgess, Shawn C.; Weis, Brian; Jones, John G.; Smith, Erin; Merritt, Matthew E.; Margolis, David; Dean Sherry, A.; Malloy, Craig R.http://www.sciencedirect.com/science/article/B6W9V-47HS4GT-7/1/a30f3e0c5749c49c708ac1a531e9026c
13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum...
Burgess, Shawn C.; Carvalho, Rui A.; Merritt, Matthew E.; Jones, John G.; Malloy, Craig R.; Sherry, A. Dean13C NMR isotopomer analysis is a powerful method for measuring metabolic fluxes through pathways intersecting in the tricarboxylic acid cycle. However, the inherent insensitivity of 13C NMR spectroscopy makes application of isotopomer analysis to small tissue samples (mouse tissue, human biopsies, or cells grown in tissue culture) problematic. 1H NMR is intrinsically more sensitive than 13C NMR and can potentia...
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