Encontrados 21 documentos, a visualizar página 1 de 3
Human adipose stem cells cell sheet constructs impact epidermal morphogenesis i...
Cerqueira, M. T.; Pirraco, R. P.; Santos, T. C.; Rodrigues, D. B.; Frias, A. M.; Martins, A.; Reis, R. L.; Marques, A. P.Among the wide range of strategies to target skin repair/regeneration, tissue engineering (TE) with stem cells at the forefront, remains as the most promising route. Cell sheet (CS) engineering is herein proposed, taking advantage of particular cell-cell and cell-extracellular matrix (ECM) interactions and subsequent cellular milieu, to create 3D TE constructs to promote full-thickness skin wound regeneration. ...
Silk-Fibroin/Methacrylated Gellan Gum Hydrogel as an novel scaffold for applica...
Pereira, H.; Correia, Joana Silva; Yan, Leping; Caridade, S. G.; Frias, A. M.; Oliveira, A. L.; Mano, J. F.; Oliveira, Joaquim M.Introduction: Knee meniscus injury is highly prevalent and there is a demand for new cost-effective treatment solutions. Tissue engineering (TE) and regenerative medicine strategies using acellular scaffolds are being used in clinical application for total or partial meniscus replacement [1]. Although this strategy has been considered as a safe and promising approach, progressive volume reduction of the implant...
Adipose stem cell-derived osteoblasts sustain the functionality of endothelial ...
Pirraco, Rogério; Ferreira, B. M.; Santos, T. C.; Frias, A. M.; Marques, A. P.; Reis, R. L.Vascularization is the most pressing issue in tissue engineering (TE) since ensuring that engineered constructs are adequately perfused after in vivo transplantation is essential for the construct’s survival. The combination of endothelial cells with current TE strategies seems the most promising approach but doubts persist as to which type of endothelial cells to use. Umbilical cord blood (UCB) cells have been...
Interfollicular epidermal stem cells : boosting and rescuing from adult skin
Cerqueira, M. T.; Frias, A. M.; Reis, R. L.; Marques, A. P.Publicado na série "Methods in Molecular Biology", vol. 989 (2013), ISSN 1064-3745 ; Epidermal stem cells isolation struggle remains, mainly due to the yet essential requirement of well-defined approaches and markers. The herein proposed methodology integrates an assemblage of strategies to accomplish the enrichment of the interfollicular epidermal stem cells multipotent fraction and their subsequent separatio...
Osteogenic differentiation of two distinct subpopulations of human adipose-deri...
Rada, Tommaso; Santos, T. C.; Marques, A. P.; Correlo, V. M.; Frias, A. M.; Castro, António G.; Neves, N. M.; Gomes, Manuela E.; Reis, R. L.The first stem cells considered for the reconstruction of bone were bone marrow mesenchymal stem cells (BMSCs). Subsequently, cells with similar marker expression panel and differentiation potential were found in new sources of cells, such as adipose tissue. This source of stem cells has a promising future in tissue-engineering applications, considering the abundance of this tissue in the human body, the easy h...
Selective cell recruitment and spatially controlled cell attachment on instruct...
Custódio, Catarina A.; Frias, A. M.; Campo, Aranzazu del; Reis, R. L.; Mano, J. F.Bioactive constructs to guide cellular mobilization and function have been proposed as an approach for a new generation of biomaterials in functional tissue engineering. Adult mesenchymal stem cells have been widely used as a source for cell based therapeutic strategies, namely tissue engineering. This is a heterogeneous cell population containing many subpopulations with distinct regenerative capacity. Thus, o...
Chitosan membranes for spatially controlled cell adhesion and specific cell rec...
Custódio, Catarina A.; Frias, A. M.; Campo, Aranzazu del; Reis, R. L.; Mano, J. F.We propose a concept of biomaterials that are able to fix specific cell types onto their surface when in contact with a mix population of cells. Adipose tissue has shown to be an interesting source of stem cells with therapeutic potential. However only a small amount of the heteroge- neous mixture of the cells extracted from lipoaspirates are stem cells, and within stem cells there are different populations wit...
Improved regeneration of mice full-thickness excisional wounds by human adipose...
Cerqueira, M. T.; Santos, T. C.; Pirraco, R. P.; Frias, A. M.; Martins, A. R.; Reis, R. L.; Marques, A. P.A totalidade dos resumos foram publicados em "J. Tissue Eng. Regen. Med. 2012; 6 (Suppl. 2) : 8–39."
Fetal stem cells obtained from amniotic fluid and wharton's jelly expanded usin...
Pinto, A. R.; Aleixo, I.; Frias, A. M.; Fernandes, S.; Rocha, L.; Reis, R. L.; Neves, N. M.Extra-embryonic tissues, such as amniotic fluid (AF) and Wharton´s Jelly (WJ) of umbilical cord, offer many advantages over both embryonic and adult stem cell sources. These tissues are routinely discarded at parturition and the extracorporeal nature of these cell sources facilitates isolation, as well as the comparatively large volume and ease of physical manipulation theoretically increases the number of stem...
Perivascular-like cells contribute to the stability of the vascular network of ...
Mendes, L. F.; Pirraco, R. P.; Szymczyk, Wojciech; Frias, A. M.; Santos, T. C.; Reis, R. L.; Marques, A. P.In recent years several studies have been supporting the existence of a close relationship in terms of function and progeny between Mesenchymal Stem Cells (MSCs) and Pericytes. This concept has opened new perspectives for the application of MSCs in Tissue Engineering (TE), with special interest for the pre-vascularization of cell dense constructs. In this work, cell sheet technology was used to create a scaffol...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||