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Diverse geno- and phenotypes of persistent listeria monocytogenes isolates from...

The persistence of Listeria monocytogenes in food-associated environments represents a key factor in transmission of this pathogen. To identify persistent and transient strains associated with production of fermented meat sausages in northern Portugal, 1,723 L. monocytogenes isolates from raw material and finished products from 11 processors were initially characterized by random amplification of polymorphic DN...

Volatile compounds contribution of Hanseniaspora guilliermondii and Hanseniaspo...

This work aims to investigate the contribution of a selected non-Saccharomyces yeast species, Hanseniaspora guilliermondii, to higher alcohols, esters, fatty acids and heavy sulphur compounds composition of red wine. Red grape must vinifications of 100 l were performed and an inoculated fermentation with H. guilliermondii was compared to a spontaneous fermentation. The presence of apiculate yeasts was observed ...

Distribution and characterization of Listeria monocytogenes clinical isolates i...

In recent years, the number of cases of listeriosis has increased worldwide. Ninety-five isolates of Listeria monocytogenes recovered from Portuguese human cases of listeriosis have been characterized by biotyping (cadmium and arsenic sensitivity), polymerase chain reaction (PCR) grouping, and by pulsed-field gel electrophoresis (PFGE) applying the enzymes AscI and ApaI. Isolates were classified into one of thr...

Genetic diversity of L. monocytogenes isolated from Portuguese cheeses

Epidemiological data on listeriosis in Portugal: 2003 – 2008

Persistence of L. monocytogenes in artisanal cheese producing plants

Cell membrane damage induced by phenolic acids on wine lactic acid bacteria

The aim of this work was to investigate the effect of phenolic acids on cell membrane permeability of lactic acid bacteria from wine. Several phenolic acids were tested for their effects on the cell membrane of Oenococcus oeni and Lactobacillus hilgardii by measuring potassium and phosphate efflux, proton influx and by assessing culture viability employing a fluorescence technique based on membrane integrity. T...

Characterization of patterns of food packaging usage in portuguese homes

This study aimed to further refine the exposure assessment of migrants from food-contact materials by characterizing, at the household level, food packaging usage (amount and type) in Portuguese urban families. Packages from domestic use were collected from a sample of 105 consumers from 34 households over a 30-day period. Collected packages (more than 6000 items) were characterized in the laboratory and data w...

Microbiological profile of salpicão de vinhais and chouriça de vinhais from raw...

Salpicão de Vinhais and Chouriça de Vinhais are traditional dry-fermented meat sausages produced in the North of Portugal. The microbiological profile during production of both products was followed in three small processing units. Lactic acid bacteria were the most prevalent microorganisms, with enhanced growth during the smoking process and a consequent decrease of pH. This study demonstrated that when highly...

Use of predictive modelling software in the evaluation of corrective actions, H...