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Genotypic and phenotypic differences between nosocomial and commensal Staphyloc...

Freitas, Ana I.; Oliveira, F.; Ramos, H.; Vasconcelos, C.; Vilanova, Manuel; Cerca, Nuno

Staphylococcus epidermidis is a common causative agent of relapsing and persistent hospital-acquired infections, not only because this bacterium belongs to the patients’ microbiota(causing autoinfection) but also because it is easily transmitted by healthcare workers (causing cross-infections). The goal of the present study was to characterize, for the first time, the biofilm-forming ability and virulence assoc...


A first new look into the interaction of Staphylococcus epidermidis biofilm-rel...

França, Ângela; Pérez-Cabezas, B.; Carvalhais, V.; Freitas, Ana I.; Pier, G.; Vilanova, Manuel; Cerca, Nuno

The widespread application of indwelling medical devices in the clinical setting, together with the remarkable ability of the commensal Staphylococcus epidermidis to adhere to these surfaces and form biofilms, has given to this bacterium the recognition of being a leading causative agent of nosocomial infections. Biofilms lifecycle is currently divided into 4 main steps: initial adhesion, accumulation, maturati...


Medium supplementation with magnesium prevented the induction of dormancy in bi...

Pérez-Cabezas, B.; Oliveira, C.; Carvalhais, V.; Freitas, Ana I.; Oliveira, F.; Cerca, Nuno; Vilanova, Manuel

Staphylococcus epidermidis is a commensal bacterium that colonizes the skin and mucous membranes, being the most prevalent staphylococcal species found in humans. However, S. epidermidis has the ability of colonize indwelling medical devices establishing biofilms, which makes this bacteria a common cause of bacteraemia particularly in immunocompromised individuals and neonates [1][2]. The presence of high amoun...


Staphylococcus epidermidis biofilm lifecycle and its virulence: from plantkonic...

França, Ângela; Freitas, Ana I.; Carvalhais, V.; Pérez-Cabezas, B.; Correia, A.; Cywes-Bentley, C.; Maira-Litrán, Tomas; Vilanova, M.; Pier, Gerald B.


Optimizing a qPCR gene expression quantification assay for S. epidermidis biofi...

França, Ângela; Freitas, Ana I.; Henriques, Ana Filipa; Cerca, Nuno

Staphylococcus epidermidis biofilm-related infections are a current concern within the medical community due to their high incidence and prevalence, particularly in patients with indwelling medical devices. Biofilm gene expression analysis by quantitative real-time PCR (qPCR) has been increasingly used to understand the role of biofilm formation in the pathogenesis of S. epidermidis infections. However, dependi...


Staphylococcus epidermidis biofilm dispersal cells : an intermediary phenotype?

França, Ângela; Freitas, Ana I.; Carvalhais, V.; Cywes-Bentley, C.; Maira-Litrán, Tomas; Vilanova, M.; Cerca, Nuno


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia