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Not All Sperm Are Equal: Functional Mitochondria Characterize a Subpopulation o...
Sousa, Ana Paula; Amaral, Alexandra; Baptista, Marta; Tavares, Renata; Caballero Campo, Pedro; Caballero Peregrín, Pedro; Freitas, AlbertinaHuman sperm samples are very heterogeneous and include a low amount of truly functional gametes. Distinct strategies have been developed to characterize and isolate this specific subpopulation. In this study we have used fluorescence microscopy and fluorescence-activated cell sorting to determine if mitochondrial function, as assessed using mitochondrialsensitive probes, could be employed as a criterion to obta...
Nuclear DNA in the determination of weighing factors to estimate exergy from or...
Fonseca, Júlio C.; Marques, João C.; Paiva, Artur A.; Freitas, Albertina M.; Madeira, Vítor M. C.; Jørgensen, Sven E.The application of ecological exergy as a suitable system-oriented development indicator of ecosystems and the estimation proposals from biomass are revised. DNA contents (C-values) of several groups of organisms are figured, either determined by flow cytometry or taken from literature. The applicability of DNA contents for determination of weighing factors to estimate ecological exergy from the biomass of orga...
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