Encontrados 4 documentos, a visualizar página 1 de 1
Protein disulphide isomerase-induced refolding of sonochemically prepared Ribon...
Fernandes, Margarida M.; Silva, Raquel; Ferreira, Helena; Donelli, Ilaria; Freddi, G.; Paulo, Artur CavacoThe present communication describes for the first time the development of Ribonuclease A (RNase A) microspheres using the sonochemical method followed by an enzymatic treatment with protein disulphide isomerase (PDI). Ultrasound application induced changes on the protein physicochemical and biological properties: the enzymatic activity of RNase A was decreased in 35% and the free thiol groups content was signif...
Insights on the mechanism of formation of protein microspheres in a biphasic sy...
Silva, Raquel; Ferreira, Helena; Azoia, Nuno G.; Shimanovich, Ulyana; Freddi, G.; Gedanken, Aharon; Paulo, Artur CavacoMicrospheres of bovine serum albumin (BSA) and silk fibroin are produced by applying ultrasound in a biphasic system consisting of an aqueous protein solution and an organic solvent. The protein microspheres are dispersed in an aqueous media where the protein remains at the interface covering the organic solvent. This only occurs when high shear forces are applied that induce changes to force the protein to the...
Enzymatic surface hydrolysis of PET : effect of structural diversity on kinetic...
Acero, Enrique Herrero; Ribitsch, D.; Steinkellner, G.; Gruber, K.; Greimel, K.; Eiteljoerg, I.; Trotscha, E.; Wei, R.; Zimmermann, W.; Zinn, M.In this study cutinases from Thermobifida cellulosilytica DSM44535 (Thc_Cut1 and Thc_Cut2) and Thermobifida fusca DSM44342 (Thf42_Cut1) hydrolyzing poly(ethylene terephthalate) (PET) were successfully cloned and expressed in E.coli BL21-Gold(DE3). Their ability to hydrolyze PET was compared with other enzymes hydrolyzing natural polyesters, including the PHA depolymerase (ePhaZmcl) from Pseudomonas fluorescens ...
Biodegradable materials based on silk fibroin and keratin
Vasconcelos, Andreia; Freddi, G.; Paulo, Artur CavacoWool and silk were dissolved and used for the preparation of blended films. Two systems are proposed: (1) blend films of silk fibroin and keratin aqueous solutions and (2) silk fibroin and keratin dissolved in formic acid. The FTIR spectra of pure films cast from aqueous solutions indicated that the keratin secondary structure mainly consists of alpha-helix and random coil conformations. The IR spectrum of pure...
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