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The Fh8 tag : a fusion partner for simple and cost-effective protein purificati...

Downstream processing is still a major bottleneck in recombinant protein production representing most of its costs. Hence, there is a continuing demand of novel and cost-effective purification processes aiming at the recovery of pure and active target protein. In this work, a novel purification methodology is presented, using the Fh8 solubility enhancer tag as fusion handle. The binding properties of Fh8 tag to...

Development of a novel fusion system for antibiotic-free Escherichia coli recom...