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Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant

Guimarães, Pedro M. R.; François, J.; Parrou, J. L.; Teixeira, J. A.; Domingues, Lucília

The construction of Saccharomyces cerevisiae strains that ferment lactose has biotechnological interest, particularly for cheese whey fermentation. A flocculent lactose-consuming S. cerevisiae recombinant expressing the LAC12 (lactose permease) and LAC4 (β-galactosidase) genes of Kluyveromyces lactis was constructed previously but showed poor efficiency in lactose fermentation. This strain was therefore subject...


Comparative transcriptome analysis between original and evolved recombinant lac...

Guimarães, Pedro M. R.; Le Berre, V.; Sokol, Serguei; François, J.; Teixeira, J. A.; Domingues, Lucília

The engineering of Saccharomyces cerevisiae strains for lactose utilization has been attempted with the intent of developing high productivity processes for alcoholic fermentation of cheese whey. A recombinant S. cerevisiae flocculent strain that efficiently ferments lactose to ethanol was previously obtained by evolutionary engineering of an original recombinant that displayed poor lactose fermentation perform...


Adaptive evolution of a lactose-consuming saccharomyces cerevisiae recombinant

Guimarães, Pedro M. R.; François, J.; Parrou, J. L.; Teixeira, J. A.; Domingues, Lucília

The construction of Saccharomyces cerevisiae strains with the ability to efficiently ferment lactose has biotechnological interest, particularly for the alcoholic fermentation of cheese whey (a high pollutant by-product of dairy industries). A flocculent lactoseconsuming S. cerevisiae recombinant expressing the LAC12 (lactose permease) and LAC4 (beta-galactosidase) genes of Kluyveromyces lactis was previously c...


Adaptive evolution of a recombinant lactose-consuming Saccharomyces cerevisiae ...

Guimarães, Pedro M. R.; Parrou, J. L.; Le Berre, V.; François, J.; Teixeira, J. A.; Domingues, Lucília

In previous work, a recombinant S. cerevisiae flocculent strain (NCYC869-A3/T1, or simply T1) with the ability to express both the LAC4 (coding for beta-galactosidase) and LAC12 (lactose permease) genes of Kluyveromyces lactis was constructed (Domingues et al., Appl Microbiol Biotechnol 51:621–626, 1999). The original recombinant obtained (T1) was able to metabolise lactose but slowly. Thus, it was subjected to...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia