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Monoclonal antibody raised against PNAG has variable effects on static S. epide...

Farnesol induces cell detachment from established S. epidermidis biofilms

Antibiotic resistance is a serious problem in Staphylococcus epidermidis infections as many clinical isolates of this organism are resistant to up to eight different antibiotics. The increased resistance to conventional antibiotic therapy has lead to the search for new antimicrobial therapeutic agents. Farnesol, an essential oil found in many plants, has been shown to be active against S. epidermidis. Using a t...

Global transcriptomic analysis of dormancy within Staphylococcus epidermidis bi...

Whole transcriptome analysis of Staphylococcus epidermidis biofilms upon contac...

Staphylococcus epidermidis is commonly associated with foreign body infections due to its ability to form biofilms on the surface of indwelling medical devices. Understanding how the biofilms form on the top of devices and interact with the host, particularly, with human blood components, is of great interest. Here we assessed the whole transcriptome of S. epidermidis biofilms upon contact with human blood. Est...

A first new look into the interaction of Staphylococcus epidermidis biofilm-rel...

The widespread application of indwelling medical devices in the clinical setting, together with the remarkable ability of the commensal Staphylococcus epidermidis to adhere to these surfaces and form biofilms, has given to this bacterium the recognition of being a leading causative agent of nosocomial infections. Biofilms lifecycle is currently divided into 4 main steps: initial adhesion, accumulation, maturati...

Functional analysis of virulence potential from Gardnerella vaginalis and other...

In the past half century, bacterial vaginosis (BV) has been a controversial topic in medical microbiology, and despite the wealth of information on this topic, the etiological agent has not yet been definitively identified [1]. The first advances on BV pointed Gardnerella vaginalis as the infectious causative agent of BV [2] but soon after it was found that G. vaginalis was also present in healthy women [3]. Ad...

Staphylococcus epidermidis biofilm lifecycle and its virulence: from plantkonic...

Variability of RNA quality extracted from biofilms of foodborne pathogens using...

The biofilm formation by foodborne pathogens is known to increase the problem related with surface disinfection procedure in the food processing environment and consequent transmission of these pathogens into the population. Messenger RNA has been increasingly used to understand the action and the consequences of disinfectants in the virulence on such biofilms. RNA quality is an important requirement for any RN...

Optimizing a qPCR gene expression quantification assay for S. epidermidis biofi...

Staphylococcus epidermidis biofilm-related infections are a current concern within the medical community due to their high incidence and prevalence, particularly in patients with indwelling medical devices. Biofilm gene expression analysis by quantitative real-time PCR (qPCR) has been increasingly used to understand the role of biofilm formation in the pathogenesis of S. epidermidis infections. However, dependi...

Farnesol antimicrobial role as biofilm cell detachment inducer in S. epidermidi...

Objetives: Farnesol is a naturally-occurring sesquiterpene that was originally isolated from essential oils found in many plants has been described to have antimicrobial potential against several bacteria, including S. epidermidis. However, farnesol mechanism of action is not yet fully understood and some contradictory findings have been reported. We recently described that while farnesol was not efficient at k...