Encontrados 9 documentos, a visualizar página 1 de 1
Benefits of spine stabilization with biodegradable scaffolds in spinal cord inj...
Silva, Nuno; Sousa, R. A.; Fraga, J. S.; Fontes, Marco; Almeida, Hugo Leite; Cerqueira, R.; Almeida, Armando; Sousa, Nuno; Reis, R. L.; Salgado, A. J.Spine stabilization upon spinal cord injury (SCI) is a standard procedure in clinical practice, but rarely employed in experimental models. Moreover, the application of biodegradable biomaterials for this would come as an advantage as it would eliminate the presence of a nondegradable prosthesis within the vertebral bone. Therefore, in the present work, we propose the use of a new biodegradable device specifica...
Unveiling the effects of the secretome of mesenchymal progenitors from the umbi...
Silva, Nuno; Rodrigues, Ana João; Sousa, Nuno; Salgado, A. J.; Fraga, J. S.; Reis, R. L.; Manadas, B.It has been previously shown that the secretome of Human Umbilical Cord Perivascular Cells (HUCPVCs), known for their mesenchymal like stem cell character, is able to increase the metabolic viability and hippocampal neuronal cell densities. However, due to the different micro-environments of the distinct brain regions it is important to study if neurons isolated from different areas have similar, or opposite, r...
Unveiling the effects of the secretome of mesenchymal progenitors from the umbi...
Fraga, J. S.; Silva, N. A.; Lourenço, A. S.; Gonçalves, V.; Neves, N. M.; Reis, R. L.; Rodrigues, A. J.; Manadas, B.; Sousa, N.; Salgado, A. J.It has been previously shown that the secretome of Human Umbilical Cord Perivascular Cells (HUCPVCs), known for their mesenchymal like stem cell character, is able to increase the metabolic viability and hippocampal neuronal cell densities. However, due to the different micro-environments of the distinct brain regions it is important to study if neurons isolated from different areas have similar, or opposite, r...
The secretome of stem cells isolated from the adipose tissue and wharton jelly ...
Ribeiro, Carlos A.; Fraga, J. S.; Grãos, Mário; Neves, N. M.; Reis, R. L.; Sousa, Nuno; Salgado, A. J.; Gimble, Jeffrey M.Introduction: It is hypothesized that administration of stromal/stem cells isolated from the adipose tissue (ASCs) and umbilical cord (HUCPVCs) can ameliorate the inured CNS. However it is still not clear whether they have similar or opposite effects on primary cultures of neuronal populations. The objective of the present work was to determine if ASCs and HUCPVCs preferentially act, or not, on specific cell po...
In vivo biodistribution of carboxymethylchitosan/poly(amidoamine) dendrimer nan...
Pereira, Vitor H.; Salgado, A. J.; Oliveira, Joaquim M.; Cerqueira, Susana R.; Frias, A. M.; Fraga, J. S.; Roque, Susana; Falcão, Ana M.Carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticles (CMCht/PAMAM) have recently been proposed for intracellular drug delivery purposes. These are constituted by a PAMAM dendrimer core grafted with chains of CMCht. Previous reports have shown that these nanoparticles disclosed an improved cytotoxic profile when compared to traditional dendrimers. Following on these results the present study aims to as...
The secretome of bone marrow mesenchymal stem cells-conditioned media varies wi...
Ribeiro, Carlos; Salgado, A. J.; Fraga, J. S.; Silva, Nuno; Reis, R. L.; Sousa, NunoTransplantation of bone marrow mesenchymal stem cells (BM-MSCs) has been shown to ameliorate the injured central nervous system (CNS). Although these effects were initially attributed to the putative differentiation of MSCs towards the neural lineage, it is now known that most of them are mediated by the secretome. Up to now most in vitro reports have dealt with the effects of the secretome on neural stem cells...
Role of human umbilical cord mesenchymal progenitors conditioned media in neuro...
Salgado, A. J.; Fraga, J. S.; Mesquita, Ana Raquel Marcelino; Neves, N. M.; Reis, R. L.; Sousa, NunoIt has been recently reported that mesenchymal progenitor/stem cells isolated from the Wharton’s Jelly (WJ) of umbilical cords (UC) ameliorate the condition of animals suffering from central nervous system (CNS)-related conditions. However, little is known on the mechanisms that regulate these actions. Therefore, the objective of the present work was to determine how the conditioned media (CM) of a population o...
Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nerv...
Salgado, A. J.; Oliveira, Joaquim M.; Pirraco, Rogério; Pereira, Vitor H.; Fraga, J. S.; Marques, A. P.; Neves, N. M.; Mano, J. F.; Reis, R. L.The applicability of CMCht/PAMAM dendrimer nanoparticles for CNS applications was investigated. AFM and TEM observations revealed that the nanoparticles possessed a nanosphere- like shape with a size from 22.0 to 30.7 nm. The nanoparticles could be bound to fluorescent-probe FITC for tracing purposes. Post-natal hippocampal neurons and cortical glial cells were both able to internalize the FITC-labeled CMCht/PA...
Effects of starch/polycaprolactone-based blends for spinal cord injury regenera...
Salgado, A. J.; Sousa, R. A.; Fraga, J. S.; Pêgo, José M.; Silva, B. A.; Malva, J. O.; Neves, N. M.; Reis, R. L.; Sousa, NunoSpinal cord injury (SCI) leads to drastic alterations on the quality of life of afflicted individuals. With the advent of Tissue Engineering and Regenerative Medicine where approaches combining biomaterials, cells and growth factors are used, one can envisage novel strategies that can adequately tackle this problem. The objective of this study was to evaluate a blend of starch with poly(ε-caprolactone) (SPCL) a...
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