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Purification, kinetics and spectral characterisation of a new versatile peroxid...
Moreira, Patrícia R.; Bouillenne, F.; Vara, E. Almeida; Malcata, F. Xavier; Frère, J.M.; Duarte, J. CardosoFrom the extracellular fluid of a novel strain of Bjerkandera sp., it was isolated, purified and identified the main enzyme responsible for Remazol Brilliant Blue R dye decolourisation. Such an enzyme is able to oxidise manganese, as well as veratryl alcohol and 2,6- dimethoxyphenol in manganese-independent reactions; hence, it can be included in the new group of versatile peroxidases. The molecular mass of sai...
Contributions to the structural characterisation of fungal versatile peroxidases
Moreira, Patrícia R.; Deharing, D.; Frère, J. M.; Malcata, F. Xavier; Duarte, J. Cardoso
Molecular characterisation of a versatile peroxidase from a bjerkandera strain
Moreira, Patrícia R.; Duez, C.; Dehareng, D.; Antunes, A.; Almeida-Vara, E.; Frère, J. M.; Malcata, F. Xavier; Duarte, J. C.The cloning and sequencing of the rbpa gene coding for a versatile peroxidase from a novel Bjerkandera strain is hereby reported. The 1777 bp isolated fragment contained a 1698 bp peroxidase-encoding gene, interrupted by 11 introns. The 367 amino acid-deduced sequence includes a 27 amino acid-signal peptide. The molecular model, built via homology modelling with crystal structures of four fungal peroxidases, hi...
Contributions to the molecular characterisation of versatile peroxidase from a ...
Moreira, Patrícia R.; Duez, C.; Antunes, A.; Almeida-Vara, E.; Frère, J. M.; Malcata, F. Xavier; Duarte, J. Cardoso
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