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Sample richness and genetic diversity as drivers of chimera formation in nSSU m...
Fonseca, V. G.; Nichols, B.; Lallias, D.; Quince, C.; Carvalho, G .R.; Power, Deborah; Creer, S.Eukaryotic diversity in environmental samples is often assessed via PCR-based amplification of nSSU genes. However, estimates of diversity derived from pyrosequencing environmental data sets are often inflated, mainly because of the formation of chimeric sequences during PCR amplification. Chimeras are hybrid products composed of distinct parental sequences that can lead to the misinterpretation of diversity es...
Data: 2012
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Origem: Sapientia - Universidade do Algarve
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