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Analysis of DNA damage and repair in Saccharomyces cerevisiae using the comet a...
Azevedo, F.; Marques, Filipe; Fokt, Hanna; Domingues, Alberta; Dias, Alberto Carlos Pires; Johansson, Björn; Oliveira, Rui Pedro Soares deIn this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. We have assessed DNA damage and repair using the comet assay, which displayed a dose-response relationship with different DNA-damaging agents. Subsequently, we used this system to assess the antigenotoxic properties of a leaf extract from Ginkgo biloba (GBE). Ex...
Measuring oxidative DNA damage and DNA repair using the yeast comet assay
Azevedo, F.; Marques, Filipe; Fokt, Hanna; Oliveira, Rui Pedro Soares de; Johansson, BjörnChromosomal DNA damage can be a result of several processes and agents of endogenous or exogenous origin. These cause strand breaks or oxidized bases that lead to strand breaks, which relax the normally supercoiled genomic DNA and increase its electrophoretic mobility. The extent of DNA damage can be assessed by single cell gel electrophoresis, where the chromosomal DNA migration distance correlates with the ex...
Analysis of DNA damage and repair in saccharomyces cerevisiae using the comet a...
Azevedo, F.; Marques, Filipe; Fokt, Hanna; Domingues, Alberta; Dias, Alberto Carlos Pires; Johansson, Björn; Oliveira, Rui Pedro Soares deIn this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. One of the goals is to use mutant strains affected in basic mechanisms of oxidative stress response and DNA repair in order to uncover the molecular targets of phytochemicals. We have assessed DNA damage and repair using the comet assay, evaluated as “comet tail...
DNA damage repair ability in cell cycle arrested saccharomyces cerevisiae cells
Fokt, Hanna; Oliveira, Rui Pedro Soares deCells are exposed permanently to different types of environmental stress and to stress caused by oxidative metabolism in mitochondria that target macromolecules like DNA. Although cells dispose a range of stress response mechanisms, balance between DNA damage-causing stress and DNA repair capacity is crucial. Cells have evolved molecular repair mechanisms in case of DNA damage so that genome stability is maint...
Actividade moduladora de extracto de Ginkgo biloba no ciclo celular de Saccharo...
Fokt, HannaDissertação de mestrado em Biotecnologia e Bio-empreendedorismo em Plantas Aromáticas e Medicinais ; Ginkgo biloba L. possui uma ampla gama de actividades biológicas e o seu extracto padronizado comercial, EGb 761, tem sido um dos produtos medicinais mais vendidos em todo o mundo, devido à sua actividade antioxidante. Efeitos benéficos sobre o sistema nervoso central, incluindo efeitos nos pacientes da doença ...
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