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A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide
Heumann, Sonja; Eberl, A.; Fischer-Colbrie, Gudrun; Pobeheim, Herbert; Kaufmann, F.; Ribitsch, D.; Paulo, Artur Cavaco; Gübitz, Georg M.An alkali stable polyamidase was isolated from a new strain of Nocardia farcinica. The enzyme consists of four subunits with a total molecular weight of 190 kDa. The polyamidase cleaved amide and ester bonds of water insoluble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hydrolyzed different soluble amides to the corresponding acid. Treatment of polyamide 6 with this...
Enzymatic surface hydrolysis of PET enhances bonding in PVC coating
Almansa, E.; Heumann, S.; Eberl, A.; Fischer-Colbrie, Gudrun; Martinkova, L.; Marek, J.; Paulo, Artur Cavaco; Guebitz, G. M.The effect of polyesterase preparations from Thermomyces lanuginosus and Beauveria brongniartii on the hydrophilicity of PET materials was assessed. As a result of polyesterase treatment the hydrophilicity of PET fabrics was increased by up to 8 cm in terms of rising height with increases in surface tension from 6.2 mNm (heat-inactivated control samples) to above 8 mNm. Both enzymes were able to increase the am...
Surface hidrolysis of polyacrylonitrile with nitrile hydrolysing enzymes from ...
Fischer-Colbrie, Gudrun; Matamá, Maria Teresa; Heumann, Sonja; Martinkova, Ludmila; Paulo, Artur Cavaco; Gübitz, Georg M.A new Micrococcus luteus strain BST20 was isolated with ability to metabolize PAN polymers as sole carbon source. Out of seven synthetized PAN copolymers containing different moieties of acrylic acid and/or vinyl acetate the polymer with lowest crystallinity (PAN with 5% vinyl acetate) was most easily metabolized. 13C labelled PAN was completely converted to the acrylic acid by this strain. M. luteus BST20 prod...
New model substrates for enzymes hydrolysing polyethyleneterephthalate and poly...
Heumann, Sonja; Eberel, Anita; Pobeheim, Herbert; Liebminger, Stefan; Fischer-Colbrie, Gudrun; Almansa, Eva; Paulo, Artur Cavaco; Gübitz, Georg M.Recently the potential of enzymes for surface hydrophilisation and/or functionalisation of polyethyleneterephthalate (PET) and polyamide (PA) has been discovered. However, there was no correlation between enzyme class/activity (e.g. esterase, lipase, cutinase) and surface hydrolysis of these polymers and consequently no simple assay to estimate this capability. Enzymes active on the model substrates bis (benzoy...
New enzymes with potential for PET surface modification
Fischer-Colbrie, Gudrun; Heumann, Sonja; Liebminger, Stefan; Almansa, Eva; Paulo, Artur CavacoThis work describes newly isolated organisms and their potential to modify the surface of polyethylene terephthalate (PET). Out of the different screening processes, four bacterial and five fungal strains were isolated. A PET model substrate was synthesized (bis (benzoyloxyethyl) terephthalate) and used in the screening process, mimicking the polymer in its crucial properties and having the advantage of defined...
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