Encontrados 28 documentos, a visualizar página 1 de 3
Validation of a PNA-FISH method for the detection of Helicobacter pylori
Cerqueira, L.; Fernandes, RM.; Ferreira, RM.; Oleastro, M.; Carneiro, F.; Brandão, C.; Pimentel-Nunes, P.; Dinis-Ribeiro, M.; Figueiredo, C.Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori cla...
Validation of a fluorescence in situ hybridization method using peptide nucleic...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisHere, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori cla...
Probe4pylori : a new kit for the rapid detection of H. pylori and associated cl...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisIn this study an evaluation of Probe4Pylori, a previously established PNA-FISH diagnostic test for H. pylori clarithromycin resistance in paraffin embedded gastric biopsies was performed and compared against culture followed by E-test and PCR. The Probe4Pylori showed very promising results with values of sensitivity (80%) and specificity (approximately 90%) for the patients harboring clarithromycin-resistant H....
Vacinação em contexto hospitalar
Figueiredo, CA apresentação intitulada “Vacinação em Contexto Hospitalar” tem como principal objectivo partilhar a experiencia do Serviço de Consulta Externa de Pediatria do hospital, no âmbito da vacinação da população-alvo da intervenção dos profissionais de saúde que integram o Serviço. O cumprimento do Plano Nacional de Vacinação (PNV) é de extrema importância e factores relacionados com o não cumprimento do PNV, justif...
Linking educational research to institutional measures of quality enhancement: ...
Huet, I.; Rafael, J.; Costa, N.; Figueiredo, C.; Oliveira, J. M.At the University of Aveiro (UA), an institutional initiative is taking place for assessing and monitoring the quality of teaching and learning in Higher Education. The Quality Assurance System (QAS) of the teaching and learning process at the UA emerges as extremely important, not only to regulate the teaching and learning process, following the quality assurance orientations at a national and international le...
Impacto da doença crónica na adolescência
Santos, S.; Santos, E.; Ferrão, A.; Figueiredo, C.RESUMO Introdução: A adolescência, as suas alterações biopsicossociais e a doença crónica influenciam-se mutuamente, com especial relevância no comportamento social, rendimento académico e comportamento sexual. Objectivos: Avaliar se a doença crónica influencia comportamentos de risco nesta idade. Material e Métodos: Estudo transversal e comparativo, realizado entre 15 de Julho e 30 de Setembro de 2008, a joven...
Imunoglobulina subcutânea: uma inovação de qualidade
Rodrigues, P; Figueiredo, C; Dantas, FAs doenças crónicas atingem hoje milhares de pessoas, de todas as faixas etárias, em todo o mundo. O início na infância pode representar um forte impacto em questões como o percurso escolar, a dinâmica familiar e a interacção social da criança. Muitas destas crianças resultarão em futuros cidadãos dependentes, incapazes de contribuir para a sociedade, representando assim uma fonte de despesa pública. A imunode...
PNA-FISH as a new diagnostic method for the determination of clarithromycin res...
Fernandes, R. M.; Cerqueira, L.; Ferreira, Rui M.; Carneiro, Fátima; Ribeiro, Mário Dinis; Figueiredo, C.; Keevil, C. W.; Azevedo, N. F.; Vieira, M. J.Triple therapy is the gold standard treatment for Helicobacter pylori eradication from the human stomach, but increased resistance to clarithromycin became the main factor of treatment failure. Until now, fastidious culturing methods are generally the method of choice to assess resistance status. In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent ...
Application of fluorescence in situ hybridisation using peptide nucleic acid pr...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Carneiro, F.; Ribeiro, M. Dinis; Figueiredo, C.; Azevedo, N. F.; Keevil, C. W.; Vieira, M. J.Microorganisms are responsible for several infectious diseases that can cause severe problems to patients and their treatment success is seriously correlated with the fast detection of the infectious agent. Some of the standard methods used, such as culturing methods are fastidious and time-consuming and do not give any information about the antibiotic resistance profile. Therefore, molecular methods have been ...
Identification of cell-surface mannans in a virulent Helicobacter pylori strain
Ferreira, José Alexandre; Azevedo, N. F.; Vieira, M. J.; Figueiredo, C.; Goodfellow, Brian J.; Monteiro, Mário A.; Coimbra, Manuel A.With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of α-(1→6)-linked mannop...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||