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A walk into the luxR regulators of actinobacteria : phylogenomic distribution a...

LuxR regulators are a widely studied group of bacterial helix-turn-helix (HTH) transcription factors involved in the regulation of many genes coding for important traits at an ecological and medical level. This regulatory family is particularly known by their involvement in quorum-sensing (QS) mechanisms, i.e., in the bacterial ability to communicate through the synthesis and binding of molecular signals. Howev...

Malic acid degradation by indigenous and commercial Saccharomyces cerevisiae wi...

Malic acid contributes to the acidic taste of wine and is, together with tartaric acid, the most abundant organic acid in wine. Contaminating lactic acid bacteria cause wine spoilage after bottling and may use malic acid as a substrate. It is therefore essential to remove excess malic acid from wines to ensure their physical, biochemical and microbial stability. The aim of this work was to gain insight in the ...

Use of two different carriers in a packed bed reactor for endopolygalacturonase...

A packed bed reactor (PBR) design was tested for the purpose of continuous pectinase production with yeasts, as a possible alternative to the traditional batch process using fungal cultures. Two different carriers - a porous glass (Siran) and a cellulosic carrier obtained from spent grains (barley) - were used to immobilize Kluyveromyces marxianus CCT 3172, a yeast strain secreting endopolygalacturonase. To imp...

Continuous production of pectinase by immobilized yeast cells on spent grains

A yeast strain secreting endopolygalacturonase was used in this work to study the possibility of continuous production of this enzyme. It is a feasible and interesting alternative to fungal batch production essentially due to the specificity of the type of pectinase excreted by Kluyveromyces marxianus CCT 3172, to the lower broth viscosity and to the easier downstream operations. In order to increase the reacto...

Acquisition of flocculation phenotype by Kluyveromyces marxianus when overexpre...

The use of flocculating yeast strains has been considered as a convenient approach to obtain high cell densities in bioreactors with increasing productivity in continuous operations. In Kluyveromyces marxianus ATTC 10022, the GAP1 gene encodes an isoform of glyceraldehyde-3-phosphate dehydrogenase–p37—that is accumulated in the cell wall and is involved in flocculation. To test the use of p37 as a tool for engi...

Molecular and physiological characterization of mono- and dicarboxylic acids pe...

Poster apresentado em XXI International Conference on Yeast Genetics and Molecular Biology, Gotemburgo, Suécia, 7 Jul. - 12 Jul. 2003

Comparative analysis of immobilization carriers for a endopolygalacturonase pro...