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Lysozyme and bovine serum albumin partitioning in polyethylene glycol–phenylala...
Silvério, Sara C.; Ferreira, Luísa A.; Martins, J. A.; Marcos, João Carlos; Macedo, E. A.; Teixeira, J. A.Polyethylene glycol (PEG) with average molecular weight of 8000 was used as a model polymer and modified by introducing an amino acid (phenylalanine) in its hydroxyl terminals. The effect of different percentages of the conjugate polymer in relation to the total polymer on binodal curve of PEG-8000–potassium phosphate buffer (KPB), pH 7.2 aqueous two-phase systems and solute partitioning was explored. The parti...
Effect of NaCl additive on properties of aqueous PEG–sodium sulfate two-phase s...
Ferreira, Luísa A.; Parpot, Pier; Teixeira, J. A.; Mikheeva, Larissa M.; Zaslavsky, Boris Y.The concentrations of all components in the phases of aqueous two-phase polyethylene glycol–sodium sulfate system of a fixed composition with different concentrations of NaCl additive were determined. Solvatochromic solvent features of aqueous media in the phases of all the systems were characterized in terms of solvent dipolarity/polarizability, solvent hydrogen bond donor acidity and hydrogen bond acceptor ba...
Salt effect on the (polyethylene glycol 8000 + sodium sulfate) aqueoustwo-phase...
Ferreira, Luísa A.; Teixeira, J. A.The relative hydrophobicity of the phases of several {polyethylene glycol (PEG) 8000 + sodium sulfate (Na2SO4)} aqueous two-phase systems (ATPSs), all containing 0.01 mol.L-1 sodium phosphate buffer (NaPB, pH 7.4) and increasing concentration of a salt additive, NaCl or KCl, up to 1.0 mol.L-1, was measured by the free energy of transfer of a methylene group between the phases, DG(CH2). The DG(CH2) of the system...
Effect of salt additives on partition of nonionic solutes in aqueous PEG–sodium...
Ferreira, Luísa A.; Teixeira, J. A.; Mikheeva, Larissa M.; Chait, Arnon; Zaslavsky, Boris Y.Partition of 12 nonionic organic compounds in aqueous PEG-8000–Na2SO4 two-phase system was examined. Effects of four salt additives (NaCl, NaSCN, NaClO4, and NaH2PO4) in the concentration range from 0.027 up to ca. 1.9 M on binodal curve of PEG-sulfate two-phase system and solute partitioning were explored. It was found that different salt additives at the relatively high concentrations display different effect...
Salt effect on the aqueous two-phase system PEG 8000-sodium sulfate
Ferreira, Luísa A.; Teixeira, J. A.The effect of added salt (NaCl or KCl) on the polyethylene glycol 8000 (PEG)−sodium sulfate (Na2SO4) aqueous two-phase system (ATPS), containing 0.01 mol·L−1 of sodium phosphate buffer (NaPB), pH 7.4, has been investigated at 296.15 K. Phase diagrams determined by the cloud point method, including tie-lines assigned from mass phase ratios according to the lever arm rule, are presented for the different PEG 8000...
Salt effect on the aqueous two-phase system PEG 8000 - sodium sulfate : physico...
Ferreira, Luísa A.; Teixeira, J. A.Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/10968
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