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Development of an immunoassay for ciprofloxacin using phage-displayed antibody ...
Gomes, Francisco Brás; Riedstra, Symon; Ferreira, A. C. Silva; Ferreira, João Paulo M.
Flavour development via lipolysis of milkfats: changes in free fatty acid pool
Regado, Mafalda A.; Cristóvão, Betina M.; Moutinho, Carla G.; Balcão, Victor M.; Aires-Barros, Raquel; Ferreira, João Paulo M.; Malcata, F. XavierUnder the trade name lipolysed milkfat (LMF), concentrated cheese flavours obtained via enzymatic treatments of cheese (or other dairy substrates) have been made available in increasing numbers. In this research effort, (anhydrous) milkfats from cows, ewes and goats were used as substrates for production of cheesy flavours. Those milkfats were subjected to modifications brought about by ten different (commercia...
Carbodiimide modification enhances activity of pig pancreatic phospholipase A2
Ferreira, João Paulo M.; Sasisekharan, Ram; Louie, Otway; Langer, RobertPig phospholipase A2, pig iso-phospholipase A2 and bovine pancreatic phospholipase A2 were reacted in solution with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, in the presence of N-hydroxysulfosuccinimide, at pH 7. The influence of micellar protectants was analyzed. In the presence of n-hexadecylphosphocholine, the losses of activity in micellar diheptanoyl-lecithin were 80, 35, and 10% in bovine phospholipa...
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