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Application of a high-throughput process analytical technology metabolomics pip...

Abstract Metabolomics aims at gathering the maximum amount of metabolic information for a total interpretation of biological systems. A process analytical technology pipeline, combining gas chromatography�mass spectrometry data preprocessing with multivariate analysis, was applied to a Port wine �forced ageing� process under different oxygen saturation regimes at 60 �C. It was found that extreme �forced ageing�...

Pheno-metabolomics characterization of a saccharomyces cerevisiae strain collec...

Genotypic and pheno-metabolomic characterization of a Saccharomyces cerevisiae ...

The objective of the present work was to gain a deeper understanding of the phenotypic diversity of natural isolates of Saccharomyces cerevisiae, using high-throughput methods in combination with bioanalytical data. A S. cerevisiae strain collection was constituted comprising 187 strains from different geographical origins and technological uses. Some microsatellite alleles and phenotypes were identified as res...

Antioxidant potential of two red seaweeds from the Brazilian coasts

In this work, in vitro antioxidant activity of two Brazilian red seaweeds, Gracilaria birdiae and Gracilaria cornea, was characterized. The total phenolic content, the radical-scavenging activity and the antioxidant activity were determined in two solvent extracts of the algae. Liquid chromatography mass spectrometry (LC MS/MS) allowed identification of important antioxidant compounds. The ethanol extract of G....

The genotypic and pheno-metabolomic landscape of a Saccharomyces cerevisiae str...

Evaluation of beer deterioration by gas chromatography–mass spectrometry/multiv...

Beer stability is a major concern for the brewing industry, as beer characteristics may be subject to significant changes during storage. This paper describes a novel non-targeted methodology for monitoring the chemical changes occurring in a lager beer exposed to accelerated aging (induced by thermal treatment: 18 days at 45 ◦C), using gas chromatography–mass spectrometry in tandem with multivariate analysis (...

Produção diferencial de compostos voláteis em caules de Pinus pinaster inoculad...

Humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost

A bacterial strain isolated from sewage sludge compost, strain SC-083T, was characterized. The isolate was a motile, Gram-positive, short rod, forming coryneform V-shaped cells during the early stages of growth. The organism was strictly aerobic and able to grow between 22 and 36 °C and between pH 5.5 and 8.0. The predominant fatty acids were cyclohexyl-C17 : 0, anteiso-C17 : 0 and iso-C16 : 0, the major respir...

New insights into a bacterial metabolic and detoxifying association responsible...

A novel pathway of molinate mineralization promoted by a defined mixed culture composed of five bacteria (named ON1 to ON5) was proposed previously. Evidence was obtained of a metabolic association between Gulosibacter molinativorax ON4T, capable of molinate breakdown, and the remaining bacteria. In the present study, the role of each isolate in that metabolic association was further explored and the possible s...

Avaliação do pico de pressão e da freqüência respiratória durante o uso de balã...

OBJETIVO: Avaliar o pico de pressão inspiratória e a freqüência ventilatória obtidos por médicos quando da utilização de balão auto-inflável neonatal em modelo de pulmão. MÉTODOS: Quinze médicos ventilaram simuladores de pulmão de recém-nascidos a termo e pré-termo, sendo os desfechos captados através de um monitor de ventilação. RESULTADOS: As medianas dos picos de pressão foram 23 (intervalo interquartil, 15-...