Whoever takes a walk among the cork oaks and pasture lands in the centre and south of Portugal will come face to face with an unquestionable reality: the abnormal loss of vegetative vigour that is apparent on many cork oaks.
In response to the need for a faster, more reliable method for identifying Phytophthora cinnamomi in cork oak soils in Portugal, a simple, fast, sensitive molecular identification method is described. It is based on a colorimetric assay which involves an oligonucleotide capture probe covalently immobilised on microtitration wells, a multi-biotinylated oligonucleotide detection probe and the PCR-amplified target...
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