Encontrados 19 documentos, a visualizar página 1 de 2
Chitosan–lignosulfonates sono-chemically prepared nanoparticles : characterisat...
Kim, Suyeon; Fernandes, Margarida M.; Matamá, Maria Teresa; Loureiro, A.; Gomes, Andreia; Paulo, Artur CavacoDue to their recognised properties of biocompatibility, biodegradability and sustainability, chitosan nanocarriers have been successfully used as new delivery systems. In this work, nanoparticles combining chitosan and lignosulfonates were developed for the first time for cosmetic and biomedical applications. The ability of lignosulfonates to act as a counter polyion for stabilisation of chitosan particles, gen...
Keratins and lipids in ethnic hair
Cruz, Célia F.; Fernandes, Margarida M.; Gomes, Andreia; Coderch, L.; Martí, M.; Méndez, Sandra; Gales, Luís; Azoia, Nuno G.; Shimanovich, UlyanaHuman hair has an important and undeniable relevance in society due to its important role in visual appearance and social communication. Hair is mainly composed of structural proteins, mainly keratin and keratin associated proteins, and lipids. Herein, we report a comprehensive study of the content and distribution of the lipids among ethnic hair, African, Asian and Caucasian hair. More interestingly, we also r...
Keratin-based peptide : biological evaluation and strengthening properties on r...
Fernandes, Margarida M.; Lima, Cristóvão F.; Loureiro, A.; Gomes, A. C.; Paulo, Artur CavacoA peptide based on a fragment of hair keratin type II cuticular protein, keratin peptide (KP), was studied as a possible strengthening agent for weakened relaxed hair. The peptide was prepared both in aqueous water formulation (WF) and organic solvent formulations (OF), to determine the effect of organic solvents on peptide interaction with hair and the differences in hair recovery. Both peptide formulations we...
Molecular modeling of hair keratin/peptide complex : using MM-PBSA calculations...
Azoia, Nuno G.; Fernandes, Margarida M.; Micaelo, N. M.; Soares, Cláudio M.; Paulo, Artur CavacoMolecular dynamics simulations of a keratin/peptide complex have been conducted to predict the binding affinity of four different peptides toward human hair. Free energy calculations on the peptides' interaction with the keratin model demonstrated that electrostatic interactions are believed to be the main driving force stabilizing the complex. The molecular mechanics–Poisson-Boltzmann surface area methodology ...
Protein disulphide isomerase-assisted functionalization of proteinaceous substr...
Fernandes, Margarida M.; Paulo, Artur CavacoProtein disulphide isomerase (PDI) is an enzyme that catalyzes thiol-disulphide exchange reactions among a broad spectrum of substrates, including proteins and low-molecular thiols and disulphides. As the first protein-folding catalyst reported, the study of PDI has mainly involved the correct folding of several cysteine-containing proteins. Its application on the functionalization of protein-based materials ha...
Protein disulphide isomerase-induced refolding of sonochemically prepared Ribon...
Fernandes, Margarida M.; Silva, Raquel; Ferreira, Helena; Donelli, Ilaria; Freddi, G.; Paulo, Artur CavacoThe present communication describes for the first time the development of Ribonuclease A (RNase A) microspheres using the sonochemical method followed by an enzymatic treatment with protein disulphide isomerase (PDI). Ultrasound application induced changes on the protein physicochemical and biological properties: the enzymatic activity of RNase A was decreased in 35% and the free thiol groups content was signif...
Protein disulphide isomerase-mediated grafting of cysteine-containing peptides ...
Fernandes, Margarida M.; Paulo, Artur CavacoThe ability of Protein disulphide isomerase (PDI) to promote the grafting of two cysteine-containing peptides onto hair was investigated in order to develop an alternative treatment for over-bleached hair. The studied peptides were designed based on human keratin and human lung surfactant proteins and were linked to a fluorescent dye to facilitate visualisation of the grafting process and to assess hair penetra...
Biology of human hair : know your hair to control it
Araújo, Rita; Fernandes, Margarida M.; Paulo, Artur Cavaco; Gomes, AndreiaHair can be engineered at different levels—its structure and surface—through modification of its constituent molecules, in particular proteins, but also the hair follicle (HF) can be genetically altered, in particular with the advent of siRNA-based applications. General aspects of hair biology are reviewed, as well as the most recent contributions to understanding hair pigmentation and the regulation of hair de...
Protein disulphide isomerase-assisted functionalization of keratin-based matrices
Fernandes, Margarida M.; Gomes, A. C.; Vasconcelos, Andreia; Munteanu, Florentina-Daniela; Tzanov, Tzanko; Gonçalves, M. Sameiro T.; End, N.In living systems, protein disulphide isomerase (PDI, EC 5.3.4.1) regulates the formation of new disulphide bonds in proteins (oxidase activity) and catalyzes the rearrangement of non-native disulphide bonds (isomerase activity), leading proteins towards their native configuration. In this study, PDI was used to attach cysteine-containing compounds (CCCs) onto hair, to enhance compound migration within hair fib...
Actividades Lúdicas para alunos do 4º Ano do 1º CEB de Língua Inglesa
Fernandes, MargaridaAs actividades lúdicas em recursos digitais e de multimédia na sensibilização para a Língua Inglesa dos alunos do 4.º ano do 1.º Ciclo do Ensino Básico vêm ao encontro das necessidades dos nossos alunos nativos digitais, bem como aos reptos lançados aos professores imigrantes digitais. Estas actividades fazem par-te da nova linguagem, a digital e de multimédia, talvez a única que os nossos alu-nos nascidos após...
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