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Structural and functional stabilization of L-asparaginase via multisubunit immo...
Balcão, Vitor M.; Mateo, Cesar; Fernández-Lafuente, R.; Malcata, F. Xavier; Guisán, José M.A new protocol for the stabilization of the quaternary structure of multimeric enzymes has been attempted using as model enzyme (tetrameric) L-asparaginase from Escherichia coli. Such strategy is based upon multisubunit covalent immobilization of the enzyme onto activated supports (agarose-glutaraldehyde). Supports activated with different densities of reactive groups were used; the higher the density of groups...
Coimmobilization of L-asparaginase and glutamate dehydrogenase onto highly acti...
Balcão, Victor M.; Mateo, Cesar; Fernández-Lafuente, R.; Malcata, F. Xavier; Guisán, José M.In the present research work, production of coimmobilized derivatives of L-asparaginase and glutamate dehydrogenase was attempted. Comparison of immobilization of each enzyme independently with coimmobilization of the two enzymes unfolded important advantages of the latter, namely a decrease in the induction period (time before the maximum reaction rate is virtually achieved) and an increase in the maximum reac...
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