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Producción de lipasas por la levadura Yarrowia lipolytica
Morín, M.; Choupina, Altino; Ferminan, E.; Burguillo, Francisco J.; Gonzalez, Francisco J.; Ruiz, L.; Dominguez, ÁngelYarrowia lipolytica es una levadura dimórfica que secreta gran cantidad de proteínas al medio de cultivo, entre ellas: proteasas - alcalinas, neutras y ácidas -, ribonucleasas, fosfatasas y lipasas. Las lipasas son enzimas importantes en el metabolismo de ácidos grasos, catalizando la hidrolisis de triglicéridos a ácidos grasos y glicerol.
The lipase system of Yarrowia lipolytica
Choupina, Altino; Gonzalez, Francisco J.; Morín, M.; Burguillo, Francisco J.; Ferminan, E.; Dominguez, ÁngelAmong yeast species, Yarrowia lipolytica is one of the highest producers of extracellular proteins ( acid, neutral and alkaline proteases, ácid phosphatase, ribonucleases and lipases). Lipases ( triacylglycerol hydrolases) are important enzymes in fat metabolism, catalyzing the breakdown of triacilglycerols to free fatty acids and glycerol. Owing to the very low solubility of ther natural substrats, this hydrol...
Non-conventional yeasts as hosts for heterologous protein production.
Domínguez, A.; Fermiñán, E.; Sánchez, M.; Gonzalez, Francisco M.; Pérez-Campo, F. M.; García, S.; Herrero, A.B.; San Vicente, A.; Cabello, J.; Prado, M.Yeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of...
Isolation, characterization and expression of genes encoding enzymes with lipas...
Choupina, Altino; Gonzalez, Francisco J.; Fermiñán, E.; Burguillo, F.J.; Domínguez, A.Yarrowia lipolytica is a dimophic yeast with very high secretory capacities and able to grow in alkanes and fatty acids as the sole carbon source. Using PCR, we have isolated two DNA fragments that after sequencing show homology with fungal lipases. By genomic DNA subcloning, we have isolated a gene nominated YlLIP1 located on Y. lipolytica chromosome IV. The gene presents an ORF of 1458 bp which encodes a puta...
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