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Epigenetic reprogramming and small RNA silencing of transposable elements in po...

Slotkin, R.K.; Vaughn, M.; Borges, F.; Tanurdzic, M.; Becker, J.D.; Feijó, J.A.; Martienssen, R.

The mutagenic activity of transposable elements (TEs) is suppressed by epigenetic silencing and small interfering RNAs (siRNAs), especially in gametes that could transmit transposed elements to the next generation. In pollen from the model plant Arabidopsis, we show that TEs are unexpectedly reactivated and transpose, but only in the pollen vegetative nucleus, which accompanies the sperm cells but does not prov...


Gametophyte interaction and sexual reproduction: how plants make a zygote

Boavida, L.C.; Vieira, A.M.; Becker, J.D.; Feijó, J.A.

The evolutionary success of higher plants relies on a very short gametophytic phase, which underlies the sexual reproduction cycle. Sexual plant reproduction takes place in special organs of the flower: pollen, the male gametophyte, is released from the anthers and then adheres, grows and interacts along various tissues of the female organs, collectively known as the pistil. Finally, it fertilizes the female ga...


Gene family analysis of the Arabidopsis pollen transcriptome reveals biological...

Pina, C.; Pinto, F.; Feijó, J.A.; Becker, J.D.

Upon germination, pollen forms a tube that elongates dramatically through female tissues to reach and fertilize ovules. While essential for the life cycle of higher plants, the genetic basis underlying most of the process is not well understood. We previously used a combination of flow cytometry sorting of viable hydrated pollen grains and GeneChip array analysis of onethird of the Arabidopsis (Arabidopsis thal...


The making of gametes in higher plants

Boavida, L.; Becker, J.D.; Feijó, J.A.

Higher plants have evolved to be one of the predominant life forms on this planet. A great deal of this evolutionary success relies in a very short gametophytic phase which underlies the sexual reproduction cycle. Sexual plant reproduction takes place in special organs of the flower. In most species the processes of gametogenesis, pollination, syngamy and embryogenesis are sequentially coordinated to give rise ...


Signalling by tips

Feijó, J.A.; Costa, S.S.; Prado, A.M.; Becker, J.D.; Certal, A.C.

New molecules, including protein kinases, lipids and molecules that have neurotransmitter activities in animals have emerged as important players in tip-growing cells. Transcriptomics analysis reveals that the largest single class of genes expressed in pollen tubes encode signal transducers, reflecting the necessity to decode complex and diverse pathways that are associated with tip growth. Many of these pathwa...


Transcriptional profiling of arabidopsis tissues reveals the Unique Characteris...

Becker, J.D.; Boavida, L.C.; Carneiro, J.; Haury, M.; Feijó, J.A.

Pollen tubes are a good model for the study of cell growth and morphogenesis because of their extreme elongation without cell division. Yet, knowledge about the genetic basis of pollen germination and tube growth is still lagging behind advances in pollen physiology and biochemistry. In an effort to reduce this gap, we have developed a new method to obtain highly purified, hydrated pollen grains of Arabidopsis ...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia