Encontrados 4 documentos, a visualizar página 1 de 1
Metabotropic glutamate and dopamine receptors co-regulate AMPA receptor activit...
Gomes, André R.; Cunha, Paulo; Nuriya, Mutsuo; Faro, Carlos J.; Huganir, Richard L.; Pires, Euclides V.; Carvalho, Ana Luísa; Duarte, Carlos B.Glutamate receptor phosphorylation has been implicated in several forms of modulation of synaptic transmission. It has been reported that protein kinase A (PKA) can phosphorylate the 03B1-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit GluR4 on Ser842, both in vitro and in vivo. Here, we studied the regulation of GluR4 phosphorylation and intracellular trafficking by PKA and by metabotrop...
The Glycosylation of the Aspartic Proteinases from Barley (Hordeum Vulgare L.) ...
Costa, Júlia; Ashford, David A.; Nimtz, Manfred; Bento, Isabel; Frazão, Carlos; Esteves, Cristina L.; Faro, Carlos J.; Kervinen, Jukka; Pires, EuclidesPlant aspartic proteinases characterised at the molecular level contain one or more consensus N-glycosylation sites [Runeberg-Roos, P., Törmäkangas, K. & Östman, A. (1991) Eur. J. Biochem. 202, 102120131027; Asakura, T., Watanabe, H., Abe, K. & Arai, S. (1995) Eur. J. Biochem. 232, 77201383; Veríssimo, P., Faro, C., Moir, A. J. G., Lin, Y., Tang, J. & Pires, E. (1996) Eur. J. Biochem. 235, 76220137681. We found...
Caseinolytic Specificity of Cardosin, an Aspartic Protease from the Cardoon Cyn...
Macedo, I. Queiroz; Faro, Carlos J.; Pires, Euclides M.The action of cardosin on bovine αs- and β-casein at 30 °C in 50 mM citrate buffer (pH 6.2) was studied. Peptides were isolated by reversed-phase HPLC on C18 columns and identified from their amino acid composition and N-terminal amino acid sequence. The relative susceptibility of peptide bonds cleaved was Phe23-Phe24 > Trp164-Tyr165 > Tyr166-Val167 > Tyr165-Tyr166 > Phe153-Tyr154 > Phe145-Tyr146 ≈ Leu149-Phe15...
Specificity and kinetics of the milk-clotting enzyme from cardoon (Cynara cardu...
Macedo, I. Queiroz; Faro, Carlos J.; Pires, Euclides M.The action of Cynara cardunculus L. protease on whole bovine K-casein, over a 3-h period at pH 6.4, was investigated. RpHPLC of the 3% trichloroacetic acid (TCA)-solublefraction of the K-casein digestion mixture showed three peptide peaks, which were identified by amino acid analysis and N-terminal analysis as the 106-169 fragment [caseinomacropeptide (CMP)]. Upon selective precipitation with 12% TCA, one glyco...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||