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On the use of dexamethasone-loaded liposomes to induce the osteogenic different...

Stem cells have received considerable attention by the scientific community because of their potential for tissue engineering and regenerative medicine. The most frequently used method to promote their differentiation is supplementation of the in vitro culture medium with growth/differentiation factors (GDFs). The limitations of that strategy caused by the short half-life of GDFs limit its efficacy in vivo and ...

Immobilization of bioactive factor-loaded liposomes at the surface of electrosp...

Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The ability to manipulate and control the surface properties is of crucial importance in the designing of scaffolds for Tissue Engineering (TE) and Regenerative Medicine. Electrospun nanofibers (NFM), due to their morphology and fibrous structure have received much attention as potential biomedical devices, TE scaf...

Cognitive appraisal as a mediator in the relationship between stress and burnout

The relation between job stressors and burnout is well established in the literature. However, the mechanisms behind this relationship are still not clear. Thus, this study has the main goal of analysing the mediating role of cognitive appraisal on the relation between occupational stress and burnout. To test this relation, structural equation modelling was used in a sample of teachers (N=333) working at a publ...

Induction of human mesenchymal stem cells osteogenesis by bioactive agent-relea...

Stem cell therapy is a rapidly evolving area of research in regenerative medicine. Mesenchymal stem cells (MSCs) have received considerable attention by the scientific community because of their potential of expansion and the ability to differentiate into various mesodermal tissues. Liposomes are well-established non-viral carrier systems, presenting significant advantages over other nanoparticle-based drug del...

Influence of conditioned medium over the chondrogenic differentiation of adult ...

The influence of patterned nanofiber meshes on human mesenchymal stem cell oste...

A specially designed electroconductive collector enables the electrospinning of P-NFM composed of areas of parallel/uniaxially aligned fibers and areas of random/orthogonal nanofiber distribution. The biological relevance of P-NFM is demonstrated using hBMSCs as an autologous cell source. The structures induce cell orientation along the uniaxially aligned fibers, mainly during earlier culturing periods under ba...

Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone rele...

Electrospun structures were proposed as scaffolds owing to their morphological and structural similarities with the extracellular matrix found in many native tissues. These !brous structures were also proposed as drug release systems by exploiting the direct dependence of the release rate of a drug on the surface area. An osteogenic differentiation factor, dexamethasone (DEX), was incorporated into electrospun ...

Biodegradable nanofibers-reinforced microfibrous composite scaffolds for bone t...

Native bone extracellular matrix (ECM) is a complex hierarchical fibrous composite structure, resulting from the assembling of collagen fibrils at several length scales, ranging from the macro to the nanoscale. The combination of nanofibers within microfibers after conventional reinforcement methodologies seems to be a feasible solution to the rational design of highly functional synthetic ECM substitutes. The ...

Cartilage tissue engineering using electrospun PCL nanofiber meshes and MSCs

Mesenchymal stem cells (MSCs) have been recognized for their ability to differentiate into cells of different tissues such as bone, cartilage, or adipose tissue, and therefore are of great interest for potential therapeutic strategies. Adherent, colony-forming, fibroblastic cells were isolated from human bone marrow aspirates, from patients undergoing knee arthroplasties, and the MSCs phenotype characterized by...

Surface modification of electrospun polycaprolactone nanofiber meshes by plasma...

A critical aspect in the development of biomaterials is the optimization of their surface properties to achieve an adequate cell response. In the present work, electrospun polycaprolactone nanofiber meshes (NFMs) are treated by radio-frequency (RF) plasma using different gases (Ar or O2), power (20 or 30 W), and exposure time (5 or 10 min). Morphological and roughness analysis show topographical changes on the ...