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Synergistic antitumoral effect of vinblastine and HSV-Tk/GCV gene therapy media...
Faneca, H.; Faustino, A.; Lima, M. C. Pedroso deDespite recent advances in conventional therapeutic approaches for cancer, the frequently observed acquired drug resistance and toxic side effects have limited their clinical application. The main goal of this work was to investigate the combined antitumoral effect of vinblastine with HSV-Tk/GCV "suicide" gene therapy mediated by human serum albumin (HSA)-associated lipoplexes, in mammary adenocarcinoma cells (...
Evaluation of the antitumoral effect mediated by IL-12 and HSV-tk genes when de...
Faneca, H.; Cabrita, A. S.; Simões, S.; Lima, M. C. Pedroso deIn the present work, we used a novel albumin-associated lipoplex formulation, containing the cationic lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (EPOPC) and cholesterol (Chol), to evaluate the antitumoral efficacy of two gene therapy strategies: immuno-gene therapy, mediated by IL-12 gene expression, and "suicide" gene therapy, mediated by HSV-tk gene expression followed by ganciclovir (GCV) tr...
Association of albumin or protamine to lipoplexes: enhancement of transfection ...
Faneca, H.; Simões, S.; Lima, M. C. Pedroso deThe successful application of gene therapy depends on the availability of carriers to efficiently deliver genetic material into target cells. Such efficacy is strongly related to key parameters including serum resistance and protection of DNA.The complexes were tested in terms of their biological activity, in the absence or presence of serum, by following transfection activity. Interaction with plasma proteins ...
Evaluation of lipid-based reagents to mediate intracellular gene delivery
Faneca, H.; Simões, S.; Lima, M. C. Pedroso deWe characterized different cationic lipid-based gene delivery systems consisting of both liposomes and nonliposomal structures, in terms of their in vitro transfection activity, resistance to the presence of serum, protective effect against nuclease degradation and stability under different storage conditions. The effect of lipid/DNA charge ratio of the resulting complexes on these properties was also evaluated...
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