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Developmental biology and cytogenetics of B. xylophilus
Hasegawa, Koichi; MOTA, Manuel; FUTAI, Kazuyoshi; MIWA, JohjiThe pine wood nematode Bursaphelenchus xylophilus reproduces bisexually: a haploid sperm fertilizes a haploid oocyte, and the two pronuclei rearrange, move together, fuse, and begin diploid development. Early embryonic events taking place in the B. xylophilus embryo are similar to those of Caenorhabditis elegans, although the anterior-posterior axis appeares to be determined oppositely to that observed for C. e...
Chromosome structure and behaviour in Bursaphelenchus xylophilus (Nematoda: Par...
HASEGAWA, Koichi; MOTA, Manuel; FUTAI, Kazuyoshi; MIWA, JohjiChromosome structure and behaviour in both meiosis of the germ cells and mitosis of the embryo from fertilisation to the two-cell stage in Bursaphelenchus xylophilus were examined by DAPI staining and three-dimensional reconstruction of serial-section images from confocal laser-scanning microscopy. By this method, each chromosome’s shape and behaviour were clearly visible in early embryogenesis from fertilisati...
Comparative Studies between Portuguese and Japanese Isolates of the Pinewood Ne...
Mota, Manuel; Takemoto, Shuhei; Takeuchi, Yuko; Hara, Naoki; Futai, KazuyoshiComparative studies between Portuguese (T and HF) and Japanese (S10, T4, C14-5 and OKD-1) isolates of the pinewood nematode Bursaphelenchus xylophilus have been made in order to provide information to better understand the possible origin of the Portuguese isolates, recently introduced in the European Union. The main comparative aspects investigated were pathogenicity (seedling mortality ratio), sexual compatib...
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