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Genetic diversity of the coat protein of olive latent virus 1 isolates
Varanda, Carla; Nolasco, Gustavo; Clara, Maria Ivone; Félix, Maria RosárioThe CP gene variability among 21 olive latent virus 1 (OLV-1) isolates obtained from different hosts and locations and at different times was assessed. Amplicons obtained by RT-PCR were cloned, and at least 10 sequences from each isolate were analyzed and compared. OLV-1 sequences available in GenBank were included. The encoded CPs consisted of 270 amino acids, except those of isolates G1S and C7 (269 aa) and G...
A degenerate pair of primers for simultaneous detection of four alphaand betane...
Varanda, Carla; Cardoso, Joana M.S.; Oliveira, Mônica; Oliveira, Solange; Clara, Maria Ivone; Félix, Maria RosárioThe high infection levels due to Olive latent virus 1 (OLV-1), Olive mild mosaic virus (OMMV) (alphanecrovirus) and Tobacco necrosis virus D (TNV-D) (betanecrovirus) in Portuguese olive orchards prompted us to develop a rapid PCR-based assay for the simultaneous detection of these viruses aimed at the sanitary selection and marketing of plant material in compliance with European Union regulations. A pair of deg...
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