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QUANTIFICAÇÃO DA PROTEÍNA CRY1AB EM FOLHAS, CAULES E GRÃOS DE DOIS HÍBRIDOS DE ...

RESUMO: Procedeu-se à avaliação do nível de infestação e respectivos danos associados à presençadas lagartas Spodoptera frugiperda e Helicoverpa zea, em dois milhos híbridos geneticamentemodificados (OGM), Dekalb DKB390 e Agroceres AG8088, que expressam a proteína Cry1Ab. Foi estabelecido em blocos casualizados, com dois fatores (hibrido e gene cry1Ab), sendo utilizadascomo controles os mesmos híbridos sem a pr...

First characterization of infectious cDNA clones of Olive mild mosaic virus

Full-length cDNA clones of an Olive mild mosaic virus (OMMV) isolate were constructed in order to find infectious cDNA clones. The sequencing of three individual full-length clones revealed some differences between them. In vitro transcription of these clones was performed and the effect of spontaneous mutations in the biological behaviour of the in vitro transcripts was evaluated by symptomatology, RNA accumul...

Biology and molecular characterization of necroviruses

Three necroviruses, family Tombusviridae, are known to infect Olea europaea L., Olive latent virus 1 (OLV-1), Olive mild mosaic virus (OMMV) and Tobacco necrosis virus D (TNV-D), often asymptomatically. OLV-1 has a wide geographical distribution whereas that of OMMVand TNV-D is not known as their discovery is recent, dating from 2005 and 2009, respectively. OMMV has received special attention because of its ori...

MÓDULO 8 - Pediatria do Internamento de Medicina e Subespecialidades

Evidence of olive mild mosaic virus transmission by Olpidium brassicae

Transmission of three strains of OMMV by an Olpidium sp. was evaluated and compared. The three strains were 1) an OMMV wild type (WT) recovered from olive trees, 2) an OMMV variant (L11) obtained after 15 serial passages of single local lesions induced in Chenopodium murale plants, and 3) a construct OMMV/OMMVL11 in which the coat protein (CP) gene replaced that of the wild type. A single-sporangial culture der...

Relação Custo Benefício na Produção de Silagem com Milho Bt

Eficiência e escala de produção são imprescindíveis para obtenção de retornos financeiros compensatórios na atividade pecuária. O aumento da produtividade demanda maior produção de energia digestível por área, a fim de suprir plenamente as exigências nutricionais dos animais ao longo do ano de forma menos dispendiosa. O custo com alimentação dos animais se eleva no período seco do ano devido à redução no cresci...

Leishmaniose visceral: experiência de um Centro Pediátrico de Referência 1990-2...

INTRODUCTION: Visceral Leishmaniasis (VL) is a systemic infection, endemic in many parts of the world, including Portugal. The aim is to review all cases of VL admitted to our hospital. PATIENTS AND METHODS: Retrospective analysis of all cases of VL admitted to a Level III Paediatric Hospital, between January 1990 and December 2009 (20 years). Demographic, epidemiological, clinical, laboratorial, therapeutic a...

Multiplex RT-PCR for detection and identification of three necroviruses that in...

An optimized multiplex RT-PCR assay was developed to discriminate three necrovirus (Olive latent virus 1 (OLV-1), Tobacco necrosis virus D (TNV-D) and Olive mild mosaic virus (OMMV)) that infect olive trees. An olive orchard consisting of 54 trees of cv. "Galega vulgar" in the south of Portugal was surveyed. dsRNA fraction was used as template and revealed the 3 viruses, singly or in multiple infections, presen...

Complete genome sequence of a Tobacco necrosis virus D isolate from olive trees

The first complete genome sequence of an isolate of Tobacco necrosis virus D (TNV-D; genus Necrovirus,family Tombusviridae) was that of a bean isolate from England. The complete nucleotide sequence of another TNV-D isolate, an Hungarian isolate from tobacco plants (TNV-DH), revealed a similar genomic organization. The single-stranded positive-sense RNA genome of TNV-D is about 3.8 kb in size and contains six op...

Extraction of bixin from annatto seeds using supercritical carbon dioxide

The solubility of 93% pure bixin in supercritical carbon dioxide (SC-CO2) and of the bixin present in annatto seeds (Bixa orellana L.) was measured. For the seeds, the measurements were made in a temperature range from 30 to 50ºC and pressure between 10 and 35 MPa and for the pure bixin, at 40ºC from 10 to 35 MPa. The main pigments of annatto seeds are bixin and norbixin, but the extracts only showed the presen...