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A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fe...
Correia, Tiago R.; Antunes, Bernardo P.; Castilho, Pedro H.; Nunes, José C.; Amorim, M. T. Pessoa de; Escobar, I. C.; Correia, I. J.; Morão, A.The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES...
Plasmid DNA recovery from fermentation broths by a combined process of micro- a...
Nunes, José C.; Morão, A.; Nunes, Catherine; Amorim, M. T. Pessoa de; Escobar, I. C.; Queiroz, J. A.Microfiltration and ultrafiltration operations were used in tandem to isolate and purify a 6050 bp plasmid DNA (pDNA). To achieve primary isolation of the plasmid from fermentation broths, immediately after cell lysis, a 0.2 μm microfiltration membrane was selected for solid/liquid separation, which was performed in a diafiltration mode, as an alternative to centrifugation. Then to attain plasmid concentration ...
Ultrafiltration of supercoiled plasmid DNA : modeling and application
Morão, A.; Nunes, J. C.; Sousa, Fani; Amorim, M. T. Pessoa de; Escobar, I. C.; Queiroz, J. A.A mass transfer model is proposed for predicting sieving coefficients, Sobs, of supercoiled plasmid DNA (pDNA), in the presence of a salt, in membranes with narrow pores, i.e., pores smaller than the gyration radii of the plasmids to be considered for a certain application. The model assumes that permeation occurs due to plasmid suction at the membrane surface as a result of the convective flow, being the proba...
Development of a model for membrane filtration of long and flexible macromolecu...
Morão, A.; Nunes, J. C.; Sousa, Fani; Amorim, M. T. Pessoa de; Escobar, I. C.; Queiroz, J. A.A model is proposed for predicting the intrinsic rejections of linear flexible molecules, of high molecular dimensions, in microfiltration and ultrafiltration membranes. The developed model is based on the available theory of hindered transport of solutes through narrow pores, assuming that convection is the only relevant transport mechanism. For the application of the model, partition coefficients of the solut...
Postsynthesis modification of a cellulose acetate ultrafiltration membrane for ...
Morão, A.; Escobar, I. C.; Amorim, M. T. Pessoa de; Lopes, A.; Gonçalves, I. C.A technique for postsynthesis modification of a cellulose acetate ultrafiltration membrane with possible application in water and wastewater treatment is studied. The technique used an oxidizing agent (persulfate) to develop free radicals on the membrane surface, and that was expected to promote grafting of hydrophilic macromolecules (polyethylene glycol). A chain-transfer agent (2-mercaptoethanol) was tested t...
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