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Comparison of plantain plantlets propagated in temporary immersion bioreactors ...
Aragón, C.; Sánchez, C.; Gonzalez-Olmedo, J.; Escalona, M.; Carvalho, L.; Amâncio, S.The current work compared the physiological characteristics of plantain (Musa AAB) plantlets micropropagated in temporary immersion bioreactors (TIB) and on a gelled medium (GM). The plantlets were evaluated during in vitro growth (in the shoot elongation phase) and at the end of ex vitro acclimatization. TIB improved rooting and gave rise to longer shoots and higher dry mass. Respiration rate was the highest a...
The physiology of ex vitro pineapple ( Ananas comosus L. Merr. var MD-2) as CAM...
Aragón, C.; Pascual, P.; González, J.; Escalona, M.; Carvalho, L.; Amâncio, S.Pineapple is one of the most important tropical crops worldwide. The use of Temporary Immersion Bioreactors (TIB) for the first stages of pineapple propagation enables precise control of plant growth, increases the rate of plant multiplication, decreases space, energy and labor requirements for pineapple plants in commercial micropropagation. Once the plantlets are ready to be taken from the reactors, they are ...
Ex vitro acclimatization of plantain plantlets micropropagated in temporary imm...
Aragón, C.; Carvalho, L.; González, J.; Escalona, M.; Amâncio, S.Plantain (Musa ABB CEMSA 3/4) plantlets were micropropagated in temporary immersion bioreactors (TIB) or in gelled medium (GM). After ex vitro transfer ROS accumulation was determined by infiltrating leaves with nitroblue tetrazolium (NBT) and 3,3’-diaminobenzidine (DAB). Stomatal cells were more stained with NBT and DAB in GM plants than in TIB plants, but the difference disappeared at the end of acclimatizati...
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