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Anestesia em oftalmologia

Descrevem-se as várias técnicas anestésicas usadas em oftalmologia. História, indicações e diferentes tipos de anestesia.

Olho seco: inovações em olho seco

Apresentam-se as características, classificação e tratamento do olho seco. Apresenta-se igualmente a suplementação alimentar como nova possibilidade no tratamento de olho seco.

Comparison of Yarrowia lipolytica and Pichia pastoris cellular response to diff...

Yeast cells exposed to adverse conditions employ a number of defense mechanisms in order to respond effectively to the stress effects of reactive oxygen species. In this work, the cellular response of Yarrowia lipolytica and Pichia pastoris to the exposure to the ROSinducing agents’ paraquat, hydrogen peroxide, and increased air pressure was analyzed. Yeast cells at exponential phase were exposed for 3 h to 1 m...

Batch and fed-batch growth of Pichia pastoris under increased air pressure

Pichia pastoris CBS 2612 behavior under air pressures of 1 bar, 3 bar and 5 bar in culture media of glycerol (pure and crude) and methanol was studied. Generally, the increase in oxygen transfer rate due to the increase of total pressure improved cellular growth for all carbon sources and for batch and fed-batch processes with different feeding rate strategies. In batch cultures, 1.4-fold, 1.2-fold, and 1.5-fol...

Erratum to : Batch and fed-batch growth of Pichia pastoris under increased air ...

Thermotoga maritima and Caldicellulosiruptor sacharolyticus co-culture for bioh...

T. maritima and C. saccharolyticus co-culture (1:3) is advantageous for hydrogen production from mixture of glucose and xylose. The higher hydrogen production observed in co-culture was associated to lower lactic acid formation.

Oxygen mass transfer rate in a pressurized lab-scale stirred bioreactor

Oxygen mass transfer from air to the liquid phase in bioreactors with aerobic cultures has long been a serious impairment to the productivity of various bioprocesses. An increase of the oxygen mass transfer rate (OTR) can be the key to overcome oxygen limitation. The influence of higher air pressure on OTR was measured and a significantly enhanced OTR could be obtained. The oxygen volumetric mass transfer coeff...

Expression of heterologous proteins by Pichia pastoris under increased air pres...

The P. pastoris expression system has gained acceptance as an important host organism for the production of heterologous proteins. Its expression system presents the advantage of the organism can grow to the desired cell density on glycerol as the carbon source and then on methanol for high level heterologous production. However, the oxygen limitation generally has a detrimental effect on the expression of for...

Bioassays against pinewood nematode: assessment of a suitable dilution agent an...

Acetone was investigated and found to be an appropriate alternative to Triton X-100 as a solvent of essential oils in bioassays aimed to investigate their effects on pinewood nematode (Bursaphelenchus xylophilus) mortality. Therefore it was used as dilution agent to screen the effectiveness of fifty two essential oils against this pest. Thirteen essential oils were highly effective, resulting in more than 90% p...

Pegada hídrica de um olival super-intensivo sob clima mediterrânico, utilizando...

A pegada hídrica de uma cultura representa o volume de água necessário para produzir, relacionando as necessidades hídricas da cultura com a produção. As suas componentes, pegadas hídricas azul, verde e cinzenta, referem-se respectivamente aos volumes de água superficial e subterrânea, precipitação e de água necessária para assimilar a poluição utilizados pela cultura. A determinação das pegadas hídricas azul e...