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Decavanadate interactions with actin: cysteine oxidation and vanadyl formation
Ramos, Susana; Duarte, Rui O.; Moura, José J. G.; Aureliano, ManuelDalton Trans., 2009, 7985–7994
Decavanadate interactions with actin: cysteine oxidation and vanadyl formation
Ramos, Susana; Duarte, Rui O.; Moura, José J. G.; Aureliano, M.Incubation of actin with decavanadate induces cysteine oxidation and oxidovanadium(IV) formation. The studies were performed combining kinetic with spectroscopic (NMR and EPR) methodologies. Although decavanadate is converted to labile oxovanadates, the rate of deoligomerization can be very slow (half-life time of 5.4 h, at 25 ◦C, with a first order kinetics), which effectively allows decavanadate to exist for ...
Sarcoplasmic reticulum calcium ATPase is inhibited by organic vanadium coordina...
Aureliano, Manuel; Henao, Fernando; Tiago, Teresa; Duarte, Rui O.; Moura, José J. G.; Baruah, Bharat; Crans, Debbie C.Inorg Chem. 2008 Jul 7;47(13):5677-84. doi: 10.1021/ic702405d ; The general affinity of the sarcoplasmic reticulum (SR) Ca (2+)-ATPase was examined for three different classes of vanadium coordination complexes including a vanadium(V) compound, pyridine-2,6-dicarboxylatodioxovanadium(V) (PDC-V(V)), and two vanadium(IV) compounds, bis(maltolato)oxovanadium(IV) (BMOV), and an analogue of amavadine, bis( N-hydrox...
NADH oxidase activity of rat and human liver xanthine oxidoreductase: potential...
Maia, Luisa; Duarte, Rui O.; Ponces-Freire, Ana; Moura, José J. G.; Mira, LurdesJ Biol Inorg Chem (2007) 12:777–787 DOI 10.1007/s00775-007-0229-7 ; To characterise the NADH oxidase activity of both xanthine dehydrogenase (XD) and xanthine oxidase (XO) forms of rat liver xanthine oxidoreductase (XOR) and to evaluate the potential role of this mammalian enzyme as an O2*- source, kinetics and electron paramagnetic resonance (EPR) spectroscopic studies were performed. A steady-state kinetics ...
Vanadium distribution, lipid peroxidation and oxidative stress markers upon dec...
Soares, S. S.; Martins, H.; Duarte, Rui O.; Moura, José J. G.; Coucelo, Josefina; Gutiérrez-Merino, Carlos; Aureliano, M.The contribution of decameric vanadate species to vanadate toxic effects in cardiac muscle was studied following an intravenous administration of a decavanadate solution (1 mM total vanadium) in Sparus aurata. Although decameric vanadate is unstable in the assay medium, it decomposes with a half-life time of 16 allowing studying its effects not only in vitro but also in vivo. After 1, 6 and 12 h upon decavanada...
Decavanadate interactions with actin: inhibition of G-actin polymerization and ...
Ramos, Susana; Manuel, Miguel; Tiago, Teresa; Duarte, Rui O.; Martins, Jorge; Gutiérrez-Merino, Carlos; Moura, José J. G.; Aureliano, M.Decameric vanadate species (V10) inhibit the rate and the extent of G-actin polymerization with an IC50 of 68 ± 22 lM and 17 ± 2 lM, respectively, whilst they induce F-actin depolymerization at a lower extent. On contrary, no effect on actin polymerization and depolymerization was detected for 2 mM concentration of ‘‘metavanadate’’ solution that contains ortho and metavanadate species, as observed by combining ...
Interactions of vanadium(V)–citrate complexes with the sarcoplasmic reticulum c...
Aureliano, M.; Tiago, Teresa; Gândara, Ricardo M. C.; Sousa, Andrea; Moderno, A.; Kaliva, M.; Salifoglou, A.; Duarte, Rui O.; Moura, José J. G.Among the biotargets interacting with vanadium is the calcium pump from the sarcoplasmic reticulum (SR). To this end, initial research efforts were launched with two vanadium(V)–citrate complexes, namely (NH4)6[V2O4(C6H4O7)2] Æ 6H2O and (NH4)6[V2O2(O2)2(C6H4O7)2] Æ 4H2O, potentially capable of interacting with the SR calcium pump by combining kinetic studies with 51V NMR spectroscopy. Upon dissolution in the re...
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