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Pheno-metabolomics characterization of a Saccharomyces cerevisiae strain collec...

Microevolutionary changes of commercial Saccharomyces cerevisiae strains recove...

Apresentação efetuada no Microbiotec2011, Braga, 2011 ; The use of commercial Saccharomyces cerevisiae wine yeast strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest time in large quantities. Within our previous work we showed that such strains are disseminated from the winery [1] and their permanence in nature induced ge...

Intra-strain phenotypic and genomic variability of the commercial Saccharomyces...

The use of commercial Saccharomyces cerevisiae wine strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest in large quantities. We showed that such strains are disseminated from the winery and their permanence in nature induced genetic changes, not found among a control group of isolates that derived from clonal expansion of ...

Study of genetic and phenotypic relationships in a Saccharomyces cerevisiae str...

Genome sequencing is essential to understand individual variation and to study the relationship between genotype and phenotype. Recently, large-scale sequencing projects of Saccharomyces cerevisiae revealed the existence of a few well defined lineages and some mosaics of that lineages, and suggested the occurrence of two domestication events during the history of association to human activities, one for sake st...

The phenotypic landscape of a Saccharomyces cerevisiae strain collection

Within our previous work [1] we developed computational models to predict strains with specific phenotypes (e.g. low ethanol resistance, growth at 30ºC and growth in media containing galactose, raffinose or urea) from microsatellite allelic patterns. The objective of the present work was to gain deeper understanding of the phenotypic diversity of a heterogeneous Saccharomyces cerevisiae strain collection, using...

Genotyping of Saccharomyces cerevisiae strains by interdelta sequence typing us...

Amplification of genomic sequences flanked by delta elements of retrotransposons TY1 and TY2 is a reliable method for characterization of Saccharomyces cerevisiae strains. The aim of this study is to evaluate the usefulness of microfluidic electrophoresis (Caliper LabChip®) to assess the factors that affect interlaboratory reproducibility of interdelta sequence typing for S. cerevisiae strain delimitation. We c...

Genetic characterization of commercial Saccharomyces cerevisiae isolates recove...

The use of commercial S. cerevisiae wine strains as fermentation starters has been extensively generalized over the past two decades. Within our previous work we showed that such strains are disseminated from the winery and their permanence in nature induced genetic changes in comparison to the commercial “mother” strain. The objective of the present study was to evaluate genome variations among four isolates o...

Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered ...

Apresentação efetuada nas 36.ª Jornadas Portuguesas de Genética, Coimbra, 2011 ; The use of commercial Saccharomyces cerevisiae wine yeast strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every year at the harvest time in large quantities. Within our previous work we showed that such strains are disseminated from the winery and can ...

Genotypic and pheno-metabolomic characterization of a Saccharomyces cerevisiae ...

The objective of the present work was to gain a deeper understanding of the phenotypic diversity of natural isolates of Saccharomyces cerevisiae, using high-throughput methods in combination with bioanalytical data. A S. cerevisiae strain collection was constituted comprising 187 strains from different geographical origins and technological uses. Some microsatellite alleles and phenotypes were identified as res...

Microevolutionary changes of commercial Saccharomyces cerevisiae strains recove...

The use of commercial Saccharomyces cerevisiae wine yeast strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest time in large quantities. Within our previous work we showed that such strains are disseminated from the winery [1] and their permanence in nature induced genetic changes that were not found among a control group o...