A PCR-RFLP assay was developed for the identification of trichodorids belonging to Nanidorus, Paratrichodorus and Trichodorus genera. Exploring the variability of the 18S SSU rDNA gene this method provides for the first time a molecular diagnosis tool alternative to the difficult and time consuming phenotyping especially of quite similar species, enabling selectivity in mixed samples with non-trichodorid specie...
Dissertação de Doutoramento em Ciências Biomédicas, área de especialização em Biologia Molecular, apresentada ao Instituto de Ciências Biomédicas de Abel Salazar da Universidade do Porto
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