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RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in G...
Sutre, AF; Sanca, A; Mané, A; Henriques, V; Portugal, C; Sancho, L; Cardoso, A; Paixão, E; Queico, CQF; Salem, JI; Antunes, A; Duarte, EL; David, SThe global tuberculosis (TB) threat is diffi cult to evaluate in countries with scarce laboratory facilities where little is known about the genetic basis of drug resistance, including the rpoB RRDR (rifampicin resistance determining region) of the Mycobacterium tuberculosis complex (MTC). The present study aimed at performing a preliminary evaluation of rpoB polymorphisms in a DNA set of MTC isolates from Guin...
Tuberculosis diagnosis after bleach processing for early stage tuberculosis lab...
David, Susana; Sutre, AF; Sanca, A; Mané, A; Henriques, V; Portugal, Clara; Sancho, Luísa; Cardoso, A; Paixão, Eleonora; Duarte, EL; Leite, CQFThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement,heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resist...
Genotyping of Portuguese MTC (Mycobacterium tuberculosis complex) animal strains
Duarte, EL; Domingos, M; Amado, A; Botelho, ADuring five years (2002-2007), 295 MTC animal isolates, representing 25% of the total isolations from the bovine TB national eradication programme, were genotyped by spoligotyping and MIRU-VNTR typing. Main purposes were to assess the discriminatory power of different typing techniques and their usefulness for the Portuguese epidemiological scenario, to confirm transmission hypothesis between different cattle h...
Mycobacterium bovis infections in Portugal: Spoligotyping and MIRU-VNTR typing ...
Duarte, EL; Domingos, M; Amado, A; Botelho, ATuberculosis is still one of the most important infectious diseases worldwide. Although human cases due to Mycobacterium bovis, the aetiological agent of bovine tuberculosis (bTB), considerably decreased in most industrialized countries, the possible zoonotic health hazard and the considerable economic losses it brings justify significant efforts to eradicate the disease in several countries. In Portugal, alth...
Molecular epidemiological links between M. Bovis strains isolated from cattle a...
Duarte, EL; Domingos, M; Amado, A; Botelho, ABovine tuberculosis (bTB) prevalence has generally been decreasing for several years in Portugal due to an ongoing UE funded eradication programme. Nevertheless, new outbreaks have been intermittently recorded in bTB free herds, especially in two Eastern regions of Portugal (Beira Interior and Portalegre District part of Alentejo region), with no evident explanation referred in official epidemiological surveys....
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