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Optimization of CO2 bio-mitigation by Chlorella vulgaris

Anjos, Mariana; Fernandes, Bruno Daniel; Vicente, A. A.; Teixeira, J. A.; Dragone, Giuliano

Abstract Biofixation of \CO2\ by microalgae has been recognized as an attractive approach to \CO2\ mitigation. The main objective of this work was to maximize the rate of \CO2\ fixation ( R \CO\ 2 ) by the green microalga Chlorella vulgaris \P12\ cultivated photoautotrophically in bubble column photobioreactors under different \CO2\ concentrations (ranging from 2% to 10%) and aeration rates (ranging from 0.1 to...


Production, chemical characterization, and sensory profile of a novel spirit el...

Sampaio, Armando; Dragone, Giuliano; Vilanova, Mar; Oliveira, J. M.; Teixeira, J. A.; Mussatto, Solange I.

This study describes a process for the production of a spirit from spent coffee ground (SCG), the chemical composition, and sensory profile of this distillate. The process consisted in three steps starting with the extraction of aroma compounds by hydrothermal treatment of SCG, followed by the fermentation of this extract supplemented with sucrose to ethanol, and the fermented broth distillation. Seventeen vola...


Relationship between starch and lipid accumulation induced by nutrient depletio...

Fernandes, Bruno Daniel; Teixeira, J. A.; Dragone, Giuliano; Vicente, A. A.; Kawano, Shigeyuki; Bišová, Katerina; Pribyl, Pavel; Zachleder, Vilém

Photosynthetic carbon partitioning into starch and neutral lipids, as well as the influence of nutrient depletion and replenishment on growth, pigments and storage compounds, were studied in the microalga, Parachlorella kessleri. Starch was utilized as a primary carbon and energy storage compound, but nutrient depletion drove the microalgae to channel fixed carbon into lipids as secondary storage compounds. Nut...


Production of high FFA Jatropa curcas oil biodiesel using oscillatory flow reactor

Supriyono, S.; Dias, J. M. M.; Dragone, Giuliano; Sulistyo, H.

Jatropa Curcas is one of the most feasible sources for biodiesel feedstock. Some advantages of Jatropa curcas oil are non edible, growing in arid soil, and high oil yield from their seed, while it’s disadvantage is tend to oxidized and form high Free Fatty Acid (FFA) due to the oil composition. Oscillatory Flow Reactor is useful when reaction should performed for a long time in a turbulence flow, the other adva...


Mixotrophic cultivation of Chlorella vulgaris using industrial dairy waste as o...

Abreu, Ana Paula; Fernandes, Bruno Daniel; Vicente, A. A.; Teixeira, J. A.; Dragone, Giuliano

Growth parameters and biochemical composition of the green microalga Chlorella vulgaris cultivated under different mixotrophic conditions were determined and compared to those obtained from a photoautotrophic control culture. Mixotrophic microalgae showed higher specific growth rate, final biomass concentration and productivities of lipids, starch and proteins than microalgae cultivated under photoautotrophic c...


Optimization of pretreatment of jatropha oil with high free fatty acids for bio...

Suwito, Supriyono; Dragone, Giuliano; Sulistyo, Hary; Murachman, Bardi; Purwono, Suryo; Teixeira, J. A.

A central composite rotatable design and response surface methodology were used in order to investigate the individual and combined effects of the ethanol-to-oil ratio, H2SO4 concentration, temperature and time of reaction on the reduction of free fatty acid (FFA) in jatropha oil. A quadratic polynomial model relating the reaction variables with FFA reduction was developed, presenting a good coefficient of dete...


Obtenção e caracterização de bebida destilada a partir da fermentação do soro d...

Dragone, Giuliano; Mussatto, Solange I.; Vilanova, Mar; Oliveira, J. M.; Teixeira, J. A.; Silva, João B. Almeida e

O presente estudo visou a produção e caracterização de uma nova bebida alcoólica através da fermentação descontínua do soro de queijo e posterior destilação do produto fermentado. Os ensaios fermentativos foram conduzidos a 35 °C e 150 rpm por 92 h, em um bioreator de 7 L (volume total) empregando a levedura Kluyveromyces fragilis. O destilado foi separado em três frações de acordo com a concentração de etan...


Brazilian kefir : structure, microbial communities and chemical composition

Magalhães, Karina Teixeira; Pereira, Gilberto V. de Melo; Campos, Cássia Roberta; Dragone, Giuliano; Schwan, Rosane F.

Microbial ecology and chemical composition of Brazilian kefir beverage was performed. The microorganisms associated with Brazilian kefir were investigated using a combination of phenotypic and genotypic methods. A total of 359 microbial isolates were identified. Lactic acid bacteria (60.5%) were the major isolated group identified, followed by yeasts (30.6%) and acetic acid bacteria (8.9%). Lactobacillus paraca...


Starch determination in Chlorella vulgaris-a comparison between acid and enzyma...

Fernandes, Bruno Daniel; Dragone, Giuliano; Abreu, Ana Paula; Geada, Pedro; Teixeira, J. A.; Vicente, A. A.

Different methods for estimating starch in Chlorella vulgaris were compared with the view of establishing a procedure suitable for rapid and accurate determination of starch content in this microalgal species. A close agreement was observed between methods that use perchloric acid and enzymatic methods that use α-amylase and amyloglucosidase to hydrolyze the starch of microalgae grown under different nitrogen c...


Comparative study of the biochemical changes and volatile compound formations d...

Magalhães, Karina Teixeira; Dragone, Giuliano; Pereira, Gilberto V. de Melo; Oliveira, J. M.; Domingues, Lucília; Teixeira, J. A.

Cheese whey (CW) and deproteinised cheese whey (DCW) were investigated for their suitability as novel substrates for the production of kefir-like beverages. Lactose consumption, ethanol production, as well as organic acids and volatile compounds formation, were determined during CW and DCW fermentation by kefir grains and compared with values obtained during the production of traditional milk kefir. The results...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia