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Protein disulphide isomerase-induced refolding of sonochemically prepared Ribon...
Fernandes, Margarida M.; Silva, Raquel; Ferreira, Helena; Donelli, Ilaria; Freddi, G.; Paulo, Artur CavacoThe present communication describes for the first time the development of Ribonuclease A (RNase A) microspheres using the sonochemical method followed by an enzymatic treatment with protein disulphide isomerase (PDI). Ultrasound application induced changes on the protein physicochemical and biological properties: the enzymatic activity of RNase A was decreased in 35% and the free thiol groups content was signif...
Data: 2012
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Origem: RepositóriUM - Universidade do Minho
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