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Sequence analysis and expression of genes involved cytoskeleton and infection m...
Choupina, Altino; Estevinho, Leticia M.; Costa, Rodrigo; Dominguez, ÁngelOomycetes from the genus Phytophthora are plant pathogens that are devastating for agriculture and natural ecosystems. The biggest productivity and yield break occurs due to the ink disease; caused by Phytophthora cinnamomi, which is one of the most widely distributed Phytophthora species, with nearly 1 000 host species. The knowledge about the molecular mechanisms responsible for its pathogenicity is an import...
Isolation and Sequencing of Actin1, Actin2 and Tubulin1 Genes Involved in Cytos...
Martins, Ivone; Lopez, Carmen; Dominguez, Ángel; Choupina, AltinoOomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. On the Nordeste Transmontano region (northeast Portugal), the Castanea sativa chestnut culture is extremely important. The biggest productivity and yield break occurs due to the ink disease, caused by Phytophthora cinnamomi which is one of the most widely distributed Phytophthora sp...
Characterization of molecular factors from plants pathogen Phytophthora cinnamo...
Choupina, Altino; Martins, Fátima; Vaz, Madalena; Dominguez, Ángel; Martins, IvoneThe culture of the chestnut tree is extremely important in the northern region of Portugal, occupying a significant proportion of useful agricultural area. The annual average chestnut production in Portugal can reach 20 000 tons. New plantation areas have increased in the last few decades. However the ink disease caused by the oomycete Phytophthora cinnamomi has damage and killed many trees and up to now no con...
Characterization of molecular factors from plants pathogen Phytophthora cinnamomi
Choupina, Altino; Martins, Fátima; Vaz, Madalena; Dominguez, Angel; Martins, I.M.The culture of the chestnut tree is extremely important in the northern region of Portugal, occupying a significant proportion of useful agricultural area. The annual average chestnut production in Portugal can reach 20 000 tons. New plantation areas have increased in the last few decades. However the ink disease caused by the oomycete Phytophthora cinnamomi has damage and killed many trees and up to now no con...
Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi
Jorge, Lurdes; Dias, Teresa; Andrade, Maria; Vaz, Madalena; Dominguez, Ángel; Choupina, AltinoActins, as the essential component of cellular microfilament, are ubiquitous and highly conserved proteins that play key roles in several basic functions of organism such as cytoskeleton morphology, cell division, cell motility, cellular signal transduction, cellular interaction and organelle movements, as well as locomotion, phagocytosis, endocytosis and exocytosis . Actins are highly conserved structural prot...
Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi
Jorge, Lurdes; Dias, Teresa; Andrade, Maria; Vaz, Madalena; Dominguez, Ángel; Choupina, AltinoActins, as the essential component of cellular microfilament, are ubiquitous and highly conserved proteins that play key roles in several basic functions of organism such as cytoskeleton morphology, cell division, cell motility, cellular signal transduction, cellular interaction and organelle movements, as well as locomotion, phagocytosis, endocytosis and exocytosis . Actins are highly conserved structural prot...
Isolation and characterization by asymmetric PCR of the ENDO1G gene for Glucan ...
Meirinho, Sofia G.; Carvalho, Marisa; Dominguez, Ángel; Choupina, AltinoInk disease is one of the most destructive diseases in Castanea sativa. The most common symptoms are root necrosies and a reduction in root growth, which invariably lead to the death of the trees. Phytophthora cinnamomi is an oomycete associated with this disease whose life cycle develops integrally in the soil. In the present work, was a fragment with 1231bp of the glucan endo-1,3-b-D-glucosidase gene obtained...
Isolation and sequence analysis of alfa-tubulin gene from Phytophora cinnamomi
Dias, Teresa; Andrade, Maria; Jorge, Lurdes; Vaz, M.; Martins, Fátima; Dominguez, Ángel; Choupina, AltinoPhytophthora diseases cause widespread economic and environmental losses worldwide. Thousands of plant species are susceptible. In Portugal, Phytophthora cinnamomi is responsible for chestnut ink disease. Despite the differences there are a number of key steps common to most infection strategies, including adhesion to the plant surface, plant penetration through the secretion of a diverse range of cell wall-deg...
Isolation and sequence analysis of alfa-tubulin gene from Phytophora cinnamomi
Dias, Teresa; Andrade, Maria; Jorge, Lurdes; Vaz, M.; Martins, Fátima; Dominguez, Ángel; Choupina, AltinoPhytophthora diseases cause widespread economic and environmental losses worldwide. Thousands of plant species are susceptible. In Portugal, Phytophthora cinnamomi is responsible for chestnut ink disease. Despite the differences there are a number of key steps common to most infection strategies, including adhesion to the plant surface, plant penetration through the secretion of a diverse range of cell wall-deg...
Identification of a Phytophthora cinnamomi glucanase inhibitor protein: a molec...
Carvalho, Marisa; Meirinho, Sofia G.; Jorge, Lurdes; Dominguez, Ángel; Choupina, AltinoThe oomycete P. cinnamomi, the causal agent of Castanea sativa ink disease, is one of the most distinctive species of Phytophthora genus, and has been associated with the decline of several forest, ornamental and fruit trees and shrubs, causing enormous economic losses worldwide. Phytophthora cell walls are composed of glucanase and have no chitin. Production of glucanase inhibitor proteins (G IPs) by Phytophth...
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