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Cell-free layer (CFL) measurements in complex geometries: contractions and bifu...
Novais, Susana; Pinho, Diana; Bento, David; Pinto, Elmano; Yaginuma, Tomoko; Fernandes, Carla S.; Garcia, Valdemar; Pereira, Ana I.; Lima, JoséIn this chapter we discuss the cell-free layer (CFL) developed adjacent to the wall of microgeometries containing complex features representative of the microcirculation, such as contractions, expansions, bifurcations and confluences. The microchannels with the different geometries were made of polydimethylsiloxane (PDMS) and we use optical techniques to evaluate the cell-free layer for red blood cells (RBC) su...
Cell-free layer measurements in a bifurcation microchannel : comparison between...
Bento, David; Pinho, Diana; Pinto, Elmano; Yaginuma, Tomoko; Correia, Teresa Montenegro; Lima, José; Pereira, Ana I.; Fernandes, Carla S.O artigo deve também estar associado ao Departamento de Electrotécnia, Tecnologia Mecânica e Química ; In the present work, in vitro blood flowing through a bifurcation microchannel was studied. The aim was to measure the Trajectories of the cell-free layer (CFL) by using different methods, i. e., a manual and two automatic methods.
Fabricação de microcanais utilizando o método de xurografia: visualização da ca...
Pinto, Elmano; Pinho, Diana; Bento, David; Correia, Teresa Montenegro; Garcia, Valdemar; Dias, Ricardo P.; Miranda, João Mário; Lima, R.No presente estudo, pretendeu-se desenvolver/melhorar uma metodologia de microfabricação de baixo custo, conhecida por xurografia e efectuar a visualização e quantificação da camada livre de células (CLC) junto às paredes para diferentes escoamentos sanguíneos em microcanais.
Blood flow in microchannels manufactured by a low cost technique: xurography
Pinto, Elmano; Taboada, B.; Faustino, V.; Cidre, Diana; Rodrigues, Raquel Oliveira; Miranda, João Mário; Garcia, Valdemar; Dias, Ricardo P.; Lima, R.The xurography is a technique that has been used to make molds to produce microchannels. In contrast to soft lithography [1, 2], xurography uses equipments and materials commonly used in the printing industry, such as cutting plotters, vinyl and other materials. The main advantage of this technique is to fabricate microchannels at a reduced cost [3, 4]. The Fahraeus-Lindqvist effect is a well know phenomenon t...
Flow of red blood cells in microchannel networks : in vitro studies
Cidre, Diana; Rodrigues, Raquel Oliveira; Faustino, V.; Pinto, Elmano; Pinho, Diana; Bento, David; Fernandes, Carla S.; Dias, Ricardo P.; Lima, R.Human blood is a multiphase biofluid primarily composed by the deformable red blood cells (RBCs) suspended in plasma. Because the complex structure of RBCs, blood exhibits unique flow characteristics on micro-scale level, due to their complex biochemical mechanisms and their response to both shear and extensional flow, which influence the rheological properties and flow behaviour of blood [1,2]. In the past yea...
Método automático para a medição da espessura camada de plasma em microcanais...
Bento, David; Cidre, Diana; Lima, José; Dias, Ricardo P.; Lima, R.Ao longo dos anos, a espessura da camada de plasma tem sido determinada com o auxilio de métodos manuais. Apesar destes métodos serem bastante fiáveis, estes são morosos e podem introduzir erros do utilizador nos dados. No presente trabalho, foi desenvolvido um método automático de processamento de imagem para a determinação da espessura camada de plasma de uma forma automática.
Flow of red blood cells in microchannel networks : in vitro studies
Cidre, Diana; Rodrigues, Raquel O.; Faustino, Vera; Pinto, Elmano; Pinho, Diana; Bento, David; Correia, Teresa; Fernandes, Carla S.; Dias, Ricardo P.Blood exhibits unique flow characteristics on micro-scale level, due to the complex biochemical structure of Red Blood Cells (RBCs) and their response to both shear and extensional flow, which influence the rheological properties and flow behavior of blood [1,2]. In the past years, several in vitro studies where made and have revealed some physiologically significant phenomena, such as Fahraeus and Fahraeus-Lin...
Flow of red blood cells in microchannel networks: in vitro studies
Cidre, Diana; Rodrigues, Raquel O.; Faustino, Vera; Pinto, Elmano; Pinho, Diana; Bento, David; Fernandes, Carla S.; Dias, Ricardo P.; Lima, R.Blood exhibits unique flow characteristics on micro-scale level, due to the complex biochemical structure of Red Blood Cells (RBCs) and their response to both shear and extensional flow, which influence the rheological properties and flow behavior of blood [1,2]. In the past years, several in vitro studies where made and have revealed some physiologically significant phenomena, such as Fahraeus and Fahraeus-Lin...
Método automático para a medição da espessura camada de plasma em microcanais ...
Bento, David; Cidre, Diana; Lima, José; Dias, Ricardo P.; Lima, RuiAo longo dos anos, a espessura da camada de plasma tem sido determinada com o auxílio de métodos manuais. Apesar destes métodos serem bastante fiáveis, estes são morosos e podem introduzir erros do utilizador nos dados. No presente trabalho, foi desenvolvido um método automático de processamento de imagem para a determinação da espessura camada de plasma de uma forma automática.
Proposal of an automatic method to measure the cell free layer in microchannels...
Bento, David; Lima, José; Dias, Ricardo P.; Lima, RuiBlood is an opaque, heterogeneous, non-Newtonian fluid composed by a yellowish homogeneous fluid – the plasma – and a series of cellular elements. Red blood cells (RBCs) in microvessels and microchannels has tendency to undergo axial migration due to the parabolic velocity profile which results in a high shear stress around wall that forces the RBCs to move towards the center induced by the tank treading motion...
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