This study aims to assess the in vivo performance of cell–scaffold constructs composed of goat marrow stromal cells (GBMCs) and SPCL (a blend of starch with polycaprolactone) fibre mesh scaffolds at different stages of development, using an autologous model. GBMCs from iliac crests were seeded onto SPCL scaffolds and in vitro cultured for 1 and 7 days in osteogenic medium. After 1 and 7 days, the constructs wer...
Values of serum tartrate-resistant acid phosphatase (TRAP)activity were obtained in adult dogs and its biological variability was assessed. Nine healthy skeletally mature Portuguese Podengo dogs were used for the determination of TRAP, total and bone alkaline phosphatase serum activities, and also to styudy their relashionship with serum minerals, namely calcium (Ca) phosphorous (P),and magnesium (Mg).The serum...
the primary objective of this work was to analyse the adequacy of the Double Cantilever Beam(DCB) test in determining fracture toughness under pure mode I loading of cortical bone tissue.A new data reduction scheme based on specimen compliance and the crack equivalent concept was used to overcome the difficulties inherent in crack monitoring during its growth. It provides a complete resistance curve, wich is fu...
Based on cyclic physiologic animal bioimpedance and body temperature a new method is being evaluated to predict estrus in dairy cattle with the aim of improving artificial insemination efficiency. Preceding in-vivo tests are being performed with a dedicated system based on the impedance converter AD5933, and a two-electrode configuration bioimplantable capsule. Acquired results will allow the optimal design of ...
Eighteen healthy skeletally mature (3 years old) ewes, with an average weight of 45 kg, of the Portuguese Churra da Terra Quente breed were used to evaluate the normal values of total and bone-specific isoform of alkaline phosphatase serum activities (ALP and BALP, respectively) and serum osteocalcin (OC) and their correlation with the serum minerals – calcium (Ca), phosphorus (P), magnesium (Mg) and ionized ca...
Recent studies suggest that bone marrow stromal cells are a potential source of osteoblasts and chondrocytes and can be used to regenerate damaged tissues using a tissue-engineering (TE) approach. However, these strategies require the use of an appropriate scaffold architecture that can support the formation de novo of either bone and cartilage tissue, or both, as in the case of osteochondral defects. The later...
Neste estudo determinou-se, numa amostragem representativa de ovelhas da raça Churra de Terra Quente, não gestantes nem lactantes e mantidas em estabulação com feno de prado ad libitum, os parâmetros hematológicos e bioquímicos gerais, assim como os electrólitos plasmáticos e as hormonas relacionadas com a função da tiróide tendo-se verificado que estes genericamente se situaram dentro dos valores de referência...
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