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Controlled RNA contamination and degradation and its impact on qPCR gene expres...

RNA quality is of utmost importance to perform gene expression quantification by qPCR. The classical methods used to determine \RNA\ quality are based on electrophoresis and spectrophotometer assessment, namely A260/A280 and A260/A230 ratios. It was previously shown that due to the complex nature of Staphylococcus epidermidis biofilms, \RNA\ extraction procedures could impact mRNA quality and thus accurate quan...

Effect of S. epidermidis biofilm matrix in RNA quality gene expression determin...