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Sources of hepatic triglyceride accumulation during high-fat feeding in the hea...

Delgado, T. C.; Pinheiro, D.; Caldeira, M.; Castro, M. M. C. A.; Geraldes, C. F. G. C.; López-Larrubia, P.; Cerdán, S.; Jones, J. G.

Hepatic triglyceride (HTG) accumulation from peripheral dietary sources and from endogenous de novo lipogenesis (DNL) was quantified in adult Sprague-Dawley rats by combining in vivo localized 1H MRS measurement of total hepatic lipid with a novel ex vivo 2H NMR analysis of HTG 2H enrichment from 2H-enriched body water. The methodology for DNL determination needs further validation against standard methodologie...


Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis o...

Delgado, T. C.; Barosa, C.; Castro, M. M. C. A.; Geraldes, C. F. G. C.; Bastos, M.; Baptista, C.; Fagulha, A.; Barros, L.; Mota, A.; Carvalheiro, M.

The contribution of gluconeogenesis to hepatic glucose production (GP) was quantified after 2H2O ingestion by Bayesian analysis of the position 2 and 5 2H-NMR signals (H2 and H5) of monoacetone glucose (MAG) derived from urinary acetaminophen glucuronide. Six controls and 10 kidney transplant (KTx) patients with cyclosporine A (CsA) immunosuppressant therapy were studied. Seven KTx patients were lean and euglyc...


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