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Inoculum type response to different pHs on bio-hydrogen production from L-arabi...

Abreu, A. A.; Danko, Anthony S.; Costa, J. C.; Ferreira, E. C.; Alves, M. M.

Biohydrogen production from arabinose was examined using four different anaerobic sludges with different pHs ranging from 4.5 to 8.0. Arabinose (30 g l−1) was used as the substrate for all experiments. Individual cumulative hydrogen production data was used to estimate the three parameters of the modified Gompertz equation. Higher hydrogen production potentials were observed for higher pH values for all the slu...


Involvement of carbon dioxide in the aerobic biodegradation of ethylene oxide, ...

Danko, Anthony S.; Freedman, David L.

The involvement of a carboxylase in metabolism of C-2 alkenes by Ochrobactrum sp. strain TD and Pseudomonas putida strain AJ was examined. With resting cells of strain TD grown on vinyl chloride, ethene, and ethylene oxide, the maximum specific rate of ethylene oxide consumption decreased significantly in the absence of external CO2 in comparison to cells provided with room air or added CO2. The amount of 14CO2...


Effect of arabinose concentration on dark fermentation hydrogen production usin...

Danko, Anthony S.; Abreu, A. A.; Alves, M. M.

Dark fermentation hydrogen production from arabinose at concentrations ranging between 0 and 100 g/L was examined in batch assays for three different mixed anaerobic cultures, two suspended sludges (S1, S2) obtained from two different sludge digesters and one granular sludge (G) obtained from a brewery wastewater treatment plant. After elimination of the methanogenic activity by heat treatment, all mixed cultur...




Effect of temperature and hydraulic retention time on hydrogen producing granul...

Abreu, A. A.; Danko, Anthony S.; Alves, M. M.

The effect of temperature and hydraulic retention time (HRT) on the homoacetogenesis and on .the morphological characteristics of hydrogen producing granules was investigated. Hydrogen was produced using an expanded granular sludge blanket (EGSB) reactor, fed with glucose and Larabinose, under mesophilic (37ºC), thermophilic (55ºC), and hyperthermophilic (70ºC) conditions. Apparent homoacetogenesis was observed...


Fermentative bio-hydrogen production from organic wastes and sugars

Abreu, A. A.; Danko, Anthony S.; Alves, J. I.; Alves, M. M.

Utilization of renewable energy has been identified as a priority within the “European Energy Policy” which stresses the need to develop new sources of green energy. A follow-up of the programme “Intelligent Energy – Europe” (Decision No. 1230/2003/EC of the European Parliament and of the Council of 26 June 2003) is projected for the 7th Framework Programme (2007-2013). The main focus of the programme is the pr...


Effect of pH on fermentative hydrogen production from L-arabinose using mixed c...

Abreu, A. A.; Danko, Anthony S.; Costa, J. C.; Alves, M. M.

Hydrogen is now considered one of the alternatives to fossil fuels. It is preferred to biogas or methane because hydrogen is not chemically bound to carbon and therefore, combustion does not contribute to green house gases or acid rain [1]. One alternative to sustainable H2 energy production from renewable energy sources is through microbiological fermentation. There have been many studies examining the effect ...


Effect of methanogenic inhibitors, inocula type, and temperature on biohydrogen...

Danko, Anthony S.; Pinheiro, F.; Abreu, A. A.; Alves, M. M.

Dark fermentation hydrogen production from a mixture of food components using two different methods of methanogenic inhibition (autoclaving and BES) and three different temperatures (37, 60, and 70 ºC) was examined in batch assays for two different mixed anaerobic cultures - one suspended sludge (S) obtained from an anaerobic digester and one granular sludge (G) obtained from a brewery wastewater treatment plan...


Bio-hydrogen production in an EGSB reactor under mesophilic, thermophilic and h...

Abreu, A. A.; Danko, Anthony S.; Alves, M. M.

Mesophilic, thermophilic and hyperthermophilic bio-hydrogen production with an expanded granular sludge blanket (EGSB) fed with glucose and arabinose, without methane production, was demonstrated. Homoacetogenesis was observed on reactor when operated under mesophilic (37ºC) conditions but not under thermophilic (55ºC) and hyper-thermophilic conditions (70ºC). It was also found that under thermophilic and hyper...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia