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Artificial cooling as an alternative to increase productivity and welfare of st...

Correa-Calderón,A.; Morales,M.; Avendaño,L.; Leyva,C.; Rivera,F.; Díaz,R.; Soto-Navarro,S.

The effects of a cooling system on productive efficiency and welfare of Holstein steers were evaluated during the summer. Sixty steers in the finishing phase were randomly allotted to one of two treatment-groups. Animals of control group were only provided with shade (non-cooled group) and a group of animals under a cooling system were installed in the shaded area (cooled group), which were operated daily from ...

Data: 2010   |   Origem: OASIS br

Testing procedures for selection of new pinewood preservation products

Nevado, B.; Diaz, R.; Santos, J.; Anjos, O.; Pestana, M.; Machado, H.

In this work we compare the results of three experimental series of tests used as a first approach on selection of natural products for wood preservation.


Chitosan/clay films' properties as affected by biopolymer and clay micro/nanopa...

Casariego, A.; Souza, B. W. S.; Cerqueira, M. A.; Teixeira, J. A.; Cruz, L.; Díaz, R.; Vicente, A. A.

Blends of chitosan (from Cuban lobster) and clay micro/nanoparticles were prepared by dispersion of the clay particles in the film matrix and the films obtained were characterized in terms of water solubility, water vapor, oxygen and carbon dioxide permeability, optical, mechanical and thermal properties using an Instron universal testing machine, differential scanning calorimetry, thermogravimetric analyses an...


Preliminary study of alternative agents to preserve Pinus pinaster wood

Anjos, O.; Diaz, R.; Gariso, L.; Machado, H.; Pestana, M.

Pine oil can be obtained from α-pinene and is one of the constituents from gun-turpentine. Is a natural product and is known for its insecticide, bactericide and fungicide proprieties. In this study dry pinewood samples cut in radial section, were impregnated with pine oil and limonene stabilised at room temperature.


Chitosan coating surface properties as affected by plasticizer, surfactant and ...

Casariego, A.; Souza, B. W. S.; Vicente, A. A.; Teixeira, J. A.; Cruz, L.; Díaz, R.

The objectives of this work were to determine the effects of the concentrations of glycerol and sorbitol (as hydrophilic plasticizers), Tween 80 (as surfactant) and chitosan on the wettability of Cuban chitosan-based edible coatings in view of their application on tomato and carrot and to develop a model allowing the optimization of coating composition. The values of the polar and dispersive components of the s...


Chitosan coating surface and permeation properties as affected by plasticizer, ...

Casariego, A.; Souza, B. W. S.; Vicente, A. A.; Teixeira, J. A.; Cruz, L.; Díaz, R.

Edible coatings can act as moisture, lipid, and gas barriers and can improve the textural properties of foods or serve as carriers of functional agents such as colors, flavors, antioxidants, nutrients and antimicrobials. The objectives of this work were to study the potentialities of chitosan (obtained from lobster of the cuban coasts) coating to extend the shelf life of fruits. To do so, the surface properties...


Induction of interleukin-10 by HIV antigens in peripheral mononuclear cells of ...

Rodrigues,D.S.S.; Brunialti,M.K.C.; Medeiros,E.A.S.; Diaz,R.S.; Turcato,G.; Salomao,R.

Evaluation of HIV-induced IL-2 production by peripheral blood mononuclear cells (PBMC) and HIV-specific T helper and cytotoxic T lymphocyte (CTL) responses in health care workers (HCW) occupationally exposed to HIV reveals a high rate of response to HIV among non-seroconverters. IL-10 is also known to interfere with HIV infection in vitro. To evaluate the induction of IL-10 by HIV antigens in HCW occupationally...

Data: 2002   |   Origem: OASIS br

Transmission of tuberculosis in Havana, Cuba: a molecular epidemiological study...

Diaz,R; Gomez,RI; Restrepo,E; Rumbaut,R; Sevy-Court,J; Valdivia,JA; van Soolingen,D

The combination of molecular and conventional epidemiological methods has improved the knowledge about the transmission of tuberculosis in urban populations. To examine transmission of tuberculosis in Havana, Cuba, with DNA fingerprinting, we studied 51 out of 92 Mycobacterium tuberculosis strains isolated from tuberculosis patients who resided in Havana and whose infection was culture-confirmed in the period f...

Data: 2001   |   Origem: OASIS br

HIV-1 subtypes among intravenous drug users from two neighboring cities in São ...

Rossini,M.A.A.; Diaz,R.S.; Caseiro,M.; Turcato,G.; Accetturi,C.A.; Sabino,E.C.

In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative ...

Data: 2001   |   Origem: OASIS br

Accuracy of replication in the polymerase chain reaction. Comparison between Th...

Diaz,R.S.; Sabino,E.C.

For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that would survive the high temperatures needed for DNA denaturation. The development of enzymes with an inherent 3' to 5' exonuclease proofreading activity, lackin...

Data: 1998   |   Origem: OASIS br

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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia