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Functional cell microcarriers : a new platform for cell separation and expansion
Custódio, Catarina A.; Cerqueira, M. T.; Marques, A. P.; Reis, R. L.; Mano, J. F.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The success of many stem cell applications in the biomedical field is highly dependent on the development of separation techniques for isolation and purification of cells with a very high yield and purity. Despite all the achievements made in the field over the past several years, new systems for effective cell sep...
Selective cell recruitment and spatially controlled cell attachment on instruct...
Custódio, Catarina A.; Frias, A. M.; Campo, Aranzazu del; Reis, R. L.; Mano, J. F.Bioactive constructs to guide cellular mobilization and function have been proposed as an approach for a new generation of biomaterials in functional tissue engineering. Adult mesenchymal stem cells have been widely used as a source for cell based therapeutic strategies, namely tissue engineering. This is a heterogeneous cell population containing many subpopulations with distinct regenerative capacity. Thus, o...
Chitosan membranes for spatially controlled cell adhesion and specific cell rec...
Custódio, Catarina A.; Frias, A. M.; Campo, Aranzazu del; Reis, R. L.; Mano, J. F.We propose a concept of biomaterials that are able to fix specific cell types onto their surface when in contact with a mix population of cells. Adipose tissue has shown to be an interesting source of stem cells with therapeutic potential. However only a small amount of the heteroge- neous mixture of the cells extracted from lipoaspirates are stem cells, and within stem cells there are different populations wit...
Multilayered polymeric particle production using superhydrophobic surfaces meth...
Lima, A. C.; Custódio, Catarina A.; Alvarez-Lorenzo, Carmen; Mano, J. F.Encapsulating technologies that render spherical particles containing cells or relevant moleculeshave been developed to be used in fields such as tissue engineering, pharmaceutics, cosmetics, agriculture, as also in other bio-related applications, namely biosensors and bioreactors. The multiple bioactive agents release, with an important role in tissue regeneration, constitutes an important strategy in tissue e...
Development of bioactive constructs through cell crosslinking for tissue regene...
Custódio, Catarina A.; Santo, Vitor E.; Gomes, Manuela E.; Reis, R. L.; Mano, J. F.Injectable systems are particularly attractive for a minimally invasive approach in tissue engineering applications. Many different methods for in situ crosslinking have been investigated however the ability of cells to promote hydrogel formation has not been fully explored. This study addressed the hypothesis that cells can promote crosslinking of chitosan microparticles forming a tridimensional network. Throu...
Self-assembled microcapsules with tunable and sustained release of macromolecul...
Costa, Rui R.; Custódio, Catarina A.; Arias, F. J.; Rodríguez-Cabello, José Carlos; Mano, J. F.Layer-by-layer (LbL) has been presented as a tool capable of constructing tunable and sustained release reservoirs for therapeutic and proliferation/differentiation agents. We report the conception of biocompatible stimuli-responsive microcapsules fabricated using LbL containing BSA as model protein. The capsules were constructed by the sequential adsorption of chitosan and a temperature-responsive elastin-like...
Layer-by-layer assembly of chitosan and recombinant biopolymers into biomimetic...
Costa, Rui R.; Custódio, Catarina A.; Arias, F. J.; Rodríguez-Cabello, José Carlos; Mano, J. F.In this work, biomimetic smart thin coatings using chitosan and a recombinant elastin-like recombinamer (ELR) containing the cell attachment sequence arginine–glycine–(aspartic acid) (RGD) are fabricated through a layer-by-layer approach. The synthetic polymer is characterized for its molecular mass and composition using mass spectroscopy and peptide sequencing. The adsorption of each polymeric layer is followe...
High-throughput evaluation of interactions between biomaterials, proteins and c...
Neto, Ana I.; Custódio, Catarina A.; Wenlong Song; Mano, J. F.We propose a new low cost platform for high-throughput analysis that permits screening the biological performance of independent combinations of biomaterials, cells and culture media. Patterned superhydrophobic flat substrates with controlled wettable spots are used to produce microarray chips for accelerated multiplexing evaluation.
Immobilization of fibronectin in chitosan substrates improves cell adhesion and...
Custódio, Catarina A.; Alves, C. M.; Reis, R. L.; Mano, J. F.Covalent grafting of biomolecules is a strategy to improve the biocompatibility and bioactivity of materials. However, it is critical to maintain the biological activity of the biomolecule upon its attachment to the surface. In the present study we compared the biological properties of chitosan, in which the surface was enriched with fibronectin (Fn), using two methodologies: chemical immobilization, using a wa...
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