Encontrados 13 documentos, a visualizar página 1 de 2
Biomimetic miniaturized platform able to sustain arrays of liquid droplets for ...
Neto, Ana I.; Correia, Clara R.; Custódio, Catarina; Mano, J. F.The development of high-throughput and combinatorial technologies is helping to speed up research that is applicable in many areas of chemistry, engineering, and biology. A new model is proposed for flat devices for the high-throughput screening of accelerated evaluations of multiplexed processes and reactions taking place in aqueous-based environments. Superhydrophobic (SH) biomimetic surfaces based on the so-...
Nanostructured hollow tubes based on chitosan and alginate multilayers
Silva, Joana M.; Duarte, Ana Rita C.; Custódio, Catarina; Sher, Praveen; Neto, Ana I.; Pinho, A. C. Marques de; Fonseca, Jaime C.; Reis, R. L.The design and production of structures with nanometer-sized polymer fi lms based on layer-by-layer (LbL) are of particular interest for tissue engineering since they allow the precise control of physical and biochemical cues of implantable devices. In this work, a method is developed for the preparation of nanostructured hollow multilayers tubes combining LbL and template leaching. The aim is to produce hollo...
Functional cell microcarriers : a new platform for cell separation and expansion
Custódio, Catarina A.; Cerqueira, M. T.; Marques, A. P.; Reis, R. L.; Mano, J. F.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The success of many stem cell applications in the biomedical field is highly dependent on the development of separation techniques for isolation and purification of cells with a very high yield and purity. Despite all the achievements made in the field over the past several years, new systems for effective cell sep...
Nanostructured hollow tubes based on chitosan and alginate multilayers
Silva, Joana M.; Duarte, Ana Rita C.; Custódio, Catarina; Sher, Praveen; Neto, Ana I.; Reis, R. L.; Mano, J. F.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The design and production of structures with nanometer-sized polymer films based on layer-by-layer (LbL) are of particular interest for tissue engineering since they allow the precise control of physical and biochemical cues, as well as the recreation of the natural complexity of ECM. In this work, we develop a met...
Miniaturized droplets-based microarray of chemical and biological high-throughp...
Neto, Ana I.; Correia, Clara R.; Custódio, Catarina; Mano, J. F.Publicado em "Journal of Tissue Engineering and Regenerative Medicine, vol. 7, supp. 1 (2013) ; The development of high-throughput and combinatorial technologies is helping to speed up research that is applicable in many areas of chemistry, engineering and biology. We propose a simple, versatile high-efficient and new superhydrophobic platform, which permits to arrange of quasi-spherical aqueous-based droplets...
Selective cell recruitment and spatially controlled cell attachment on instruct...
Custódio, Catarina A.; Frias, A. M.; Campo, Aranzazu del; Reis, R. L.; Mano, J. F.Bioactive constructs to guide cellular mobilization and function have been proposed as an approach for a new generation of biomaterials in functional tissue engineering. Adult mesenchymal stem cells have been widely used as a source for cell based therapeutic strategies, namely tissue engineering. This is a heterogeneous cell population containing many subpopulations with distinct regenerative capacity. Thus, o...
Chitosan membranes for spatially controlled cell adhesion and specific cell rec...
Custódio, Catarina A.; Frias, A. M.; Campo, Aranzazu del; Reis, R. L.; Mano, J. F.We propose a concept of biomaterials that are able to fix specific cell types onto their surface when in contact with a mix population of cells. Adipose tissue has shown to be an interesting source of stem cells with therapeutic potential. However only a small amount of the heteroge- neous mixture of the cells extracted from lipoaspirates are stem cells, and within stem cells there are different populations wit...
Multilayered polymeric particle production using superhydrophobic surfaces meth...
Lima, A. C.; Custódio, Catarina A.; Alvarez-Lorenzo, Carmen; Mano, J. F.Encapsulating technologies that render spherical particles containing cells or relevant moleculeshave been developed to be used in fields such as tissue engineering, pharmaceutics, cosmetics, agriculture, as also in other bio-related applications, namely biosensors and bioreactors. The multiple bioactive agents release, with an important role in tissue regeneration, constitutes an important strategy in tissue e...
Development of bioactive constructs through cell crosslinking for tissue regene...
Custódio, Catarina A.; Santo, Vitor E.; Gomes, Manuela E.; Reis, R. L.; Mano, J. F.Injectable systems are particularly attractive for a minimally invasive approach in tissue engineering applications. Many different methods for in situ crosslinking have been investigated however the ability of cells to promote hydrogel formation has not been fully explored. This study addressed the hypothesis that cells can promote crosslinking of chitosan microparticles forming a tridimensional network. Throu...
Self-assembled microcapsules with tunable and sustained release of macromolecul...
Costa, Rui R.; Custódio, Catarina A.; Arias, F. J.; Rodríguez-Cabello, José Carlos; Mano, J. F.Layer-by-layer (LbL) has been presented as a tool capable of constructing tunable and sustained release reservoirs for therapeutic and proliferation/differentiation agents. We report the conception of biocompatible stimuli-responsive microcapsules fabricated using LbL containing BSA as model protein. The capsules were constructed by the sequential adsorption of chitosan and a temperature-responsive elastin-like...
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